Rabbit anti Human CD279 antibody detects CD279, a co-stimulatory molecule also known as programmed cell death-1 (PD-1). CD279 is a single-pass type 1 membrane protein belonging to the CD28 family, and functions mainly as a negative regulator of T-cell activation. CD279 has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance. CD279 is inducibly expressed by T-cells, B-cells, NK-T-cells and monocytes upon activation. Loss of CD279 function has been associated with a number of autoimmune diseases, including rheumatoid arthritis, type I diabetes and ankylosing spondylitis. CD279 may be targeted therapeutically in the treatment of HIV infection to reduce T-cell exhaustion (Freeman et al. 2006).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human CD279 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- A peptide corresponding to a 16 amino acid sequence from near the centre of Human CD279.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Q15116 Related reagents
- Entrez Gene
- PDCD1 Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0004725 protein tyrosine phosphatase activity
- GO:0004871 signal transducer activity
- GO:0006959 humoral immune response
- GO:0007275 multicellular organismal development
- GO:0031295 T cell costimulation
- For research purposes only
Applications of CD279 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||5.0ug/ml|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human brain tissue
- Western Blotting
- AHP1706 detects a band of approximately 45kDa in THP-1 cell lysate
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Antigen Retrieval Buffer, pH8.0||BUF025A||500 ml||P|
|Antigen Retrieval Buffer, pH8.0||BUF025C||50 ml||P|
|TidyBlot™ Western Blot Detection Reagent:HRP||STAR209P||0.5 ml||WB*|
|Histar Detection System||STAR3000A||50 Tests||C P|
Product Specific References
Ishida, Y. et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
EMBO J. 11 (11): 3887-95.
Zhong, X. et al. (2004) Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger.
Int Immunol. 16 (8): 1181-8.
Nishimura, H. et al. (1999) Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.