Find out more about A.I.den - our SearchBot

CD279 antibody

100% Secure
Anti Human CD279 Antibody thumbnail image 1 Anti Human CD279 Antibody thumbnail image 2
Anti Human CD279 Antibody gallery image 1
Western blot analysis of whole cell lysate from THP-1 promonocytic leukemia cells with Rabbit anti Human CD279 (AHP1706) in the absence (A) or presence (B) of blocking peptide
Anti Human CD279 Antibody gallery image 2
Immunohistochemical staining of human brain with Rabbit anti Human CD279 (AHP1706)

Rabbit anti Human CD279

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1706
Datasheet
P* WB
SDS
0.1 mg loader

Rabbit anti Human CD279 antibody detects CD279, a co-stimulatory molecule also known as programmed cell death-1 (PD-1). CD279 is a single-pass type 1 membrane protein belonging to the CD28 family, and functions mainly as a negative regulator of T-cell activation. CD279 has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance. CD279 is inducibly expressed by T-cells, B-cells, NK-T-cells and monocytes upon activation. Loss of CD279 function has been associated with a number of autoimmune diseases, including rheumatoid arthritis, type I diabetes and ankylosing spondylitis. CD279 may be targeted therapeutically in the treatment of HIV infection to reduce T-cell exhaustion (Freeman et al. 2006).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human CD279 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 16 amino acid sequence from near the centre of Human CD279.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q15116
Entrez Gene
PDCD1
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0004725 protein tyrosine phosphatase activity
GO:0004871 signal transducer activity
GO:0006959 humoral immune response
GO:0007275 multicellular organismal development
GO:0031295 T cell costimulation
Regulatory
For research purposes only

Applications of CD279 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5.0ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain tissue
Western Blotting
AHP1706 detects a band of approximately 45kDa in THP-1 cell lysate
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

Further Reading

  1. Ishida, Y. et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
    EMBO J. 11 (11): 3887-95.
  2. Zhong, X. et al. (2004) Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger.
    Int Immunol. 16 (8): 1181-8.
  3. Nishimura, H. et al. (1999) Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
    Immunity 11:141-51.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
View more products with CD279 specificity