CD271 antibody
Rabbit anti CD271
CD271 antibody recognizes CD271 also known as tumor necrosis factor receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor or low affinity neurotrophin receptor p75NTR. This antibody has been extensively validated for western blotting using whole cell lysates.
CD271 Antibody with Control Lysate
CD271 antibody recognizes CD271 also known as tumor necrosis factor receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor or low affinity neurotrophin receptor p75NTR. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00240 | WB | 100 µl | ||||
| VPA00240KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CD271 antibody recognizes the CD271 antigen, also known as tumor necrosis factor (TNF) receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor, low affinity neurotrophin receptor p75NTR and low-affinity nerve growth factor receptor, p75 ICD.
CD271 contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain (provided by RefSeq, Jul 2008).
Rabbit anti Human CD271 antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
CD271 contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain (provided by RefSeq, Jul 2008).
Rabbit anti Human CD271 antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CD271 detects a band of approximately 75 kDa in Raji cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 276-310 amino acids from the C-terminal region of human CD271
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Raji lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P08138 Related reagents
- Entrez Gene
- NGFR Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005887 integral to plasma membrane
- GO:0005654 nucleoplasm
- GO:0005768 endosome
- GO:0006916 anti-apoptosis
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009986 cell surface
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0045786 negative regulation of cell cycle
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050771 negative regulation of axonogenesis
- GO:0050772 positive regulation of axonogenesis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CD271 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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