CD271 antibody

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Rabbit anti CD271

CD271 antibody recognizes CD271 also known as tumor necrosis factor receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor or low affinity neurotrophin receptor p75NTR. This antibody has been extensively validated for western blotting using whole cell lysates.

CD271 Antibody with Control Lysate

CD271 antibody recognizes CD271 also known as tumor necrosis factor receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor or low affinity neurotrophin receptor p75NTR. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00240 WB 100 µl
VPA00240KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CD271 antibody recognizes the CD271 antigen, also known as tumor necrosis factor (TNF) receptor superfamily member 16, NGF receptor, low affinity nerve growth factor receptor, low affinity neurotrophin receptor p75NTR and low-affinity nerve growth factor receptor, p75 ICD.

CD271 contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain (provided by RefSeq, Jul 2008).

Rabbit anti Human CD271 antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD271 detects a band of approximately 75 kDa in Raji cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 276-310 amino acids from the C-terminal region of human CD271
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08138 Related reagents
Entrez Gene
NGFR Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005887 integral to plasma membrane
GO:0005654 nucleoplasm
GO:0005768 endosome
GO:0006916 anti-apoptosis
GO:0008624 induction of apoptosis by extracellular signals
GO:0009986 cell surface
GO:0031293 membrane protein intracellular domain proteolysis
GO:0045786 negative regulation of cell cycle
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050771 negative regulation of axonogenesis
GO:0050772 positive regulation of axonogenesis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD271 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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