CD271 antibody

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Anti Human CD271 Antibody thumbnail image 2

Anti Human CD271 Antibody gallery image 1 — Western blot analysis of 25(A), 50(B) and 100(C) ng purified recombinant human nerve growth factor receptor (NGFR) with Rabbit anti Human CD271 (AHP1014)

Anti Human CD271 Antibody gallery image 2 — Immunocytochemical staining of A20 mouse B-cell lymphoma cells with Rabbit anti Human CD271 (AHP1014)

Rabbit anti Human CD271

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1014	C WB			0.1 mg

Rabbit anti Human CD271 antibody recognises CD271, a transmembrane protein of ~70-75 kDa, otherwise known as Nerve Growth Factor Receptor (NGFR). It is a pro-apoptotic member of the tumour necrosis factor receptor (TNFR) family and its ligands include NGF, brain derived growth neurotrophic factor (BDNF), NT3 and NT4. CD271 has been shown to interact with tropomyosin-receptor-kinase A (TrkA) forming the high-affinity NGF receptor, while CD271 can signal alone, constituting the low-affinity NGF receptor. When signalling alone, CD271 is involved in apoptosis, neurite outgrowth during development and myelination. Mutations and changes in CD271 expression have been implicated in various pathological diseases including Alzheimer’s Disease, cerebral oedema and retinoblastoma.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to CD271 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide (NaN₃)

Immunogen

Recombinant human CD271

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P08138 Related reagents
Entrez Gene: NGFR Related reagents
GO Terms: GO:0006915 apoptosis; GO:0005576 extracellular region; GO:0005829 cytosol; GO:0005887 integral to plasma membrane; GO:0005654 nucleoplasm; GO:0005768 endosome; GO:0006916 anti-apoptosis; GO:0008624 induction of apoptosis by extracellular signals; GO:0009986 cell surface; GO:0031293 membrane protein intracellular domain proteolysis; GO:0045786 negative regulation of cell cycle; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050771 negative regulation of axonogenesis; GO:0050772 positive regulation of axonogenesis
Regulatory: For research purposes only

Applications of CD271 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Western Blotting			1ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1014 detects a band of approximately 75-80kDa in A-20 cell lysate.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*