CD271 antibody

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Rabbit anti Human CD271

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1014 C WB 0.1 mg
Rabbit anti Human CD271 antibody recognises CD271, a transmembrane protein of ~70-75 kDa, otherwise known as Nerve Growth Factor Receptor (NGFR). It is a pro-apoptotic member of the tumour necrosis factor receptor (TNFR) family and its ligands include NGF, brain derived growth neurotrophic factor (BDNF), NT3 and NT4. CD271 has been shown to interact with tropomyosin-receptor-kinase A (TrkA) forming the high-affinity NGF receptor, while CD271 can signal alone, constituting the low-affinity NGF receptor. When signalling alone, CD271 is involved in apoptosis, neurite outgrowth during development and myelination. Mutations and changes in CD271 expression have been implicated in various pathological diseases including Alzheimer’s Disease, cerebral oedema and retinoblastoma.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD271 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Recombinant human CD271
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08138 Related reagents
Entrez Gene
NGFR Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005887 integral to plasma membrane
GO:0005654 nucleoplasm
GO:0005768 endosome
GO:0006916 anti-apoptosis
GO:0008624 induction of apoptosis by extracellular signals
GO:0009986 cell surface
GO:0031293 membrane protein intracellular domain proteolysis
GO:0045786 negative regulation of cell cycle
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050771 negative regulation of axonogenesis
GO:0050772 positive regulation of axonogenesis
Regulatory
For research purposes only

Applications of CD271 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1014 detects a band of approximately 75-80kDa in A-20 cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Johnson, D. et al. (1986) Expression and structure of the human NGF receptor.
    Cell. 47 (4): 545-54.
  2. Nykjaer, A. et al. (2005) p75NTR--live or let die.
    Curr Opin Neurobiol. 15 (1): 49-57.
  3. Schor, N.F. (2005) The p75 neurotrophin receptor in human development and disease.
    Prog Neurobiol. 77 (3): 201-14.