CD265 (soluble) antibody

100% Secure

Rabbit anti Human CD265 (soluble):Preservative Free

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Rabbit anti Human CD265 (soluble) antibody, recognizes CD265 also known as RANK, TNFRSFIIA, osteoclast differentiation factor receptor and receptor activator of NF-κB. This antibody recognizes the soluble TNFR homologous cysteine rich portion of the extracellular domain of the CD265 receptor.

CD265 has major functions in bone remodeling, signaling to regulate osteoclast formation, activation and survival in normal bone modeling and remodeling in an array of pathological conditions characterized by upregulated bone turnover (Boyce & Xing 2008). CD265 signaling is induced upon binding of its ligand RANKL which results in the recruitment of one of several adapter proteins including TRAF6. This can result in the activation of the NF-κB, MAPK or c-Src signaling pathways (Nagy & Penninger 2015).

Beyond its crucial function in osteoclastogenesis, CD265 has also been implicated in other physiological processes including immunotolerance, organogenesis of the immune system and osteoimmunology (Sigl et al. 2016).

Product Details

Target Species
Product Form
Purified IgG - lyophilized
Reconstitute with 0.1 ml PBS + 0.1% BSA
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant human sRANK and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
E. coli derived recombinant human soluble CD265 containing the TNFR-homologous, cysteine-rich portion of the extracellular domain of RANK receptor
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0002250 adaptive immune response
GO:0002548 monocyte chemotaxis
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0046872 metal ion binding
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0009897 external side of plasma membrane
GO:0030316 osteoclast differentiation
GO:0032496 response to lipopolysaccharide
GO:0034612 response to tumor necrosis factor
GO:0043507 positive regulation of JUN kinase activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060086 circadian temperature homeostasis
GO:0070555 response to interleukin-1
GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
GO:0071848 positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
For research purposes only

Applications of CD265 (soluble) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra