CD265 (soluble) antibody

Rabbit anti Human CD265 (soluble):Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD265 (soluble)

Product Code Applications Pack Size List Price Your Price Qty
AHP2797Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
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loader
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Rabbit anti Human CD265 (soluble) antibody, recognizes CD265 also known as RANK, TNFRSFIIA, osteoclast differentiation factor receptor and receptor activator of NF-κB. This antibody recognizes the soluble TNFR homologous cysteine rich portion of the extracellular domain of the CD265 receptor.

CD265 has major functions in bone remodeling, signaling to regulate osteoclast formation, activation and survival in normal bone modeling and remodeling in an array of pathological conditions characterized by upregulated bone turnover (Boyce & Xing 2008). CD265 signaling is induced upon binding of its ligand RANKL which results in the recruitment of one of several adapter proteins including TRAF6. This can result in the activation of the NF-κB, MAPK or c-Src signaling pathways (Nagy & Penninger 2015).

Beyond its crucial function in osteoclastogenesis, CD265 has also been implicated in other physiological processes including immunotolerance, organogenesis of the immune system and osteoimmunology (Sigl et al. 2016).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml PBS + 0.1% BSA
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant human sRANK and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant human soluble CD265 containing the TNFR-homologous, cysteine-rich portion of the extracellular domain of RANK receptor
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
Srank
UniProt
Q9Y6Q6
Entrez Gene
TNFRSF11A
GO Terms
GO:0002250 adaptive immune response
GO:0002548 monocyte chemotaxis
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0046872 metal ion binding
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0009897 external side of plasma membrane
GO:0030316 osteoclast differentiation
GO:0032496 response to lipopolysaccharide
GO:0034612 response to tumor necrosis factor
GO:0043507 positive regulation of JUN kinase activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060086 circadian temperature homeostasis
GO:0070555 response to interleukin-1
GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
GO:0071848 positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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