CD265 (soluble) antibody
Rabbit anti Human CD265 (soluble) antibody, recognizes CD265 also known as RANK, TNFRSFIIA, osteoclast differentiation factor receptor and receptor activator of NF-κB. This antibody recognizes the soluble TNFR homologous cysteine rich portion of the extracellular domain of the CD265 receptor.
CD265 has major functions in bone remodeling, signaling to regulate osteoclast formation, activation and survival in normal bone modeling and remodeling in an array of pathological conditions characterized by upregulated bone turnover (Boyce & Xing 2008). CD265 signaling is induced upon binding of its ligand RANKL which results in the recruitment of one of several adapter proteins including TRAF6. This can result in the activation of the NF-κB, MAPK or c-Src signaling pathways (Nagy & Penninger 2015).
Beyond its crucial function in osteoclastogenesis, CD265 has also been implicated in other physiological processes including immunotolerance, organogenesis of the immune system and osteoimmunology (Sigl et al. 2016).
CD265 has major functions in bone remodeling, signaling to regulate osteoclast formation, activation and survival in normal bone modeling and remodeling in an array of pathological conditions characterized by upregulated bone turnover (Boyce & Xing 2008). CD265 signaling is induced upon binding of its ligand RANKL which results in the recruitment of one of several adapter proteins including TRAF6. This can result in the activation of the NF-κB, MAPK or c-Src signaling pathways (Nagy & Penninger 2015).
Beyond its crucial function in osteoclastogenesis, CD265 has also been implicated in other physiological processes including immunotolerance, organogenesis of the immune system and osteoimmunology (Sigl et al. 2016).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml PBS + 0.1% BSA
- Antiserum Preparation
- Produced from sera of rabbits immunized with recombinant human sRANK and purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human soluble CD265 containing the TNFR-homologous, cysteine-rich portion of the extracellular domain of RANK receptor
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 0.1 ng/μg of protein (< 1 EU/μg)
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y6Q6
- Entrez Gene
- TNFRSF11A
- GO Terms
- GO:0002250 adaptive immune response
- GO:0002548 monocyte chemotaxis
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0046872 metal ion binding
- GO:0007267 cell-cell signaling
- GO:0008284 positive regulation of cell proliferation
- GO:0009897 external side of plasma membrane
- GO:0030316 osteoclast differentiation
- GO:0032496 response to lipopolysaccharide
- GO:0034612 response to tumor necrosis factor
- GO:0043507 positive regulation of JUN kinase activity
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0060086 circadian temperature homeostasis
- GO:0070555 response to interleukin-1
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
- GO:0071848 positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
- Regulatory
- For research purposes only
Applications of CD265 (soluble) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 ug/ml | 2.0 ug/ml | |
| Western Blotting | 0.1 ug/ml | 0.2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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