CD254 antibody



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P ResetRabbit anti Human CD254
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD254
Rabbit anti Human CD254 antibody recognizes the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein. CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis. CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CD254.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.5ug/ml | |
Immunohistology - Paraffin 1 | ![]() |
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Western Blotting | ![]() |
0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Description | Sheep anti Rabbit IgG:FITC | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Sheep anti Rabbit IgG:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 | ||||||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
Further Reading
-
Boyle, W.J. et al. (2003) Osteoclast differentiation and activation.
Nature. 423 (6937): 337-42.
- Synonyms
- sRANKL
- RRID
- AB_2206063
- UniProt
- O14788
- Entrez Gene
- TNFSF11
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006955 immune response
- GO:0030316 osteoclast differentiation
- View More GO Terms
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0034112 positive regulation of homotypic cell-cell adhesion
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0045780 positive regulation of bone resorption
- GO:0050870 positive regulation of T cell activation
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051466 positive regulation of corticotropin-releasing hormone secretion
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
- GO:0071848 positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
AHP1209

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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