CD253 antibody

Rabbit anti Human CD253

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD253

Product Code Applications Pack Size List Price Your Price Qty
AHP1215
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SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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Rabbit anti Human CD253 antibody recognizes human tumor necrosis factor ligand superfamily member 10 otherwise known as TRAIL, CD253 or Apo2L. CD253 is a 281 amino acid type II transmembrane glycoprotein and member of the tumour necrosis factor superfamily, which induces apoptosis in tumour and transformed cells, but not normal cells.

Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.

CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human CD253 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography against immobilized antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human CD253 (PHP125)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 150ng/ml
Immunohistology - Paraffin 1 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Metastatic carcinoma of lymph node from breast tissue

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References for CD253 antibody

  1. Koyama, S. et al. (2002) Expression of TNF-related apoptosis-inducing ligand (TRAIL) and its receptors in gastric carcinoma and tumor-infiltrating lymphocytes: a possible mechanism of immune evasion of the tumor.
    J Cancer Res Clin Oncol. 128 (2): 73-9.
  2. Plasilova, M. et al. (2002) TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.
    Leukemia. 16 (1): 67-73.
  3. Graf, D. et al. (2003) Inhibition of taurolithocholate 3-sulfate-induced apoptosis by cyclic AMP in rat hepatocytes involves protein kinase A-dependent and -independent mechanisms.
    Arch Biochem Biophys. 415 (1): 34-42.
  4. Chi, H.C. et al. (2012) Thyroid hormone receptors promote metastasis of human hepatoma cells via regulation of TRAIL.
    Cell Death Differ. 19 (11): 1802-14.

Further Reading

  1. Almasan, A. & Ashkenazi, A. (2003) Apo2L/TRAIL: apoptosis signaling, biology, and potential for cancer therapy.
    Cytokine Growth Factor Rev. 14 (3-4): 337-48.

RRID
AB_871973
UniProt
P50591
Entrez Gene
TNFSF10
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0046872 metal ion binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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