CD243 antibody

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Rabbit anti Human CD243

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1311 P* 50 µg
Rabbit anti Human CD243 antibody detects CD243, also known as MDR1 (multi-drug resistance protein 1) and P-glycoprotein. CD243 is a ~170 kDa membrane-associated protein which functions as an ATP-dependent drug efflux pump with broad substrate specificity.

CD243 is highly expressed in the kidney, liver, adrenal gland and intestine, suggesting a primary role in the secretion of metabolites into bile, urine and the lumen of the intestinal tract. CD243 also prevents the accumulation of toxic compounds in critical organs such as the brain, gonads, and bone marrow, and also functions in the placenta to protect the foetus.

CD243 is rapidly up-regulated by chemotherapeutic drugs and is a well established mediator of multidrug resistance.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD243 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide: cytoplasmic domain of human CD243, conjugated to keyhole limpet hemocyanin.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08183 Related reagents
Entrez Gene
ABCB1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0008559 xenobiotic-transporting ATPase activity
GO:0009986 cell surface
GO:0016324 apical plasma membrane
GO:0046581 intercellular canaliculus
Regulatory
For research purposes only

Applications of CD243 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 20ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Adrenal, cortex.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

Further Reading

  1. Marzolini, C. et al. (2004) Polymorphisms in human MDR1 (P-glycoprotein): recent advances and clinical relevance.
    Clin. Pharmacol. Ther. 75: 13-33.