CD230 antibody

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Goat anti Human CD230 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP498 E P WB 0.1 ml
Goat anti Human CD230 antibody recognizes human Major prion protein, also known as PrP27-30, CD230, or PrP33-35C. CD230 is a 253 amino acid ~27 kDa GPI anchored membrane protein. CD230 is found in large amounts in the brain of patients with transmissible spongiform encephalopathies (TSEs) or prion diseases, fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.

CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.

Goat anti Human CD230 (N-Terminal) antibody has been shown to stain PrPSc plaques in formalin fixed sections of CJD brain. It also reacts with PrPc.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
Hamster
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human PrP27-30 were raised by repeated immunisation of goats with highly purified antigen.
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 79-97 of the N-terminus of human prion protein

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04156 Related reagents
Entrez Gene
PRNP Related reagents
Regulatory
For research purposes only

Applications of CD230 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/30,000
Immunohistology - Paraffin 1/200
Western Blotting 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
CJD brain

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for CD230 antibody

  1. Shinagawa, M. et al. (1986) Immunoreactivity of a synthetic pentadecapeptide corresponding to the N-terminal region of the scrapie prion protein.
    J Gen Virol. 67 ( Pt 8): 1745-50.
  2. Mao-Draayer, Y.et al. (2002) Emerging patterns of diffusion-weighted MR imaging in Creutzfeldt-Jakob disease: case report and review of the literature.
    AJNR Am J Neuroradiol. 23 (4): 550-6.