CD226 antibody

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Rabbit anti CD226

Rabbit anti human CD226 antibody recognizes the CD226 antigen, also known as DNAX accessory molecule 1, DNAM1, PTA1 or T cell activation antigen 1. This antibody has been extensively validated for western blotting using whole cell lysates.

CD226 Antibody with Control Lysate

Rabbit anti human CD226 antibody recognizes the CD226 antigen, also known as DNAX accessory molecule 1, DNAM1, PTA1 or T cell activation antigen 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00420 WB 100 µl
VPA00420KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CD226 antibody recognizes CD226, also known as DNAX accessory molecule 1, T lineage-specific activation antigen 1 antigen, PTA1 and T cell activation antigen 1.

The CD226 antigen is a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation (provided by RefSeq, Jul 2008).

Rabbit anti Human CD226 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 307-335 of human CD226

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q15762 Related reagents
Entrez Gene
CD226 Related reagents
GO Terms
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0002891 positive regulation of immunoglobulin mediated immune response
GO:0007155 cell adhesion
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0008037 cell recognition
GO:0009986 cell surface
GO:0019901 protein kinase binding
GO:0033005 positive regulation of mast cell activation
GO:0045121 membrane raft
GO:0060369 positive regulation of Fc receptor mediated stimulatory signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CD226 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti CD226 detects a band of approximately 34 kDa in Raji cell lysate
Western Blotting
Anti CD226 detects a band of approximately 34 kDa in Raji cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB