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CD220 antibody

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Anti Human CD220 Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human islets of Langerhans with Rabbit anti Human CD220 (AHP1216).

Rabbit anti Human CD220

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1216
Datasheet
P*
SDS
50 µg loader

Rabbit anti Human CD220 antibody detects an epitope within in the internal domain of human CD220, also known as Insulin receptor (INRS). CD220 is a heterotetrameric membrane glycoprotein consisting of four disulfide linked subunits; two alpha subunits and two beta subunits. CD220 is a member of the tyrosine kinase family of receptors, and mediates the effects of insulin and IGF-I (insulin-like growth factor-I) through the activation of insulin receptor substrate (IRS) proteins and downstream effector cascades.

Activation of CD220 leads to a number of cellular effects, including glucose uptake, the regulation of cell proliferation and gene expression. These effects mostly occur in skeletal muscle, fat and the liver. Neuronal CD220 signalling has been shown to have an important role in energy homeostasis, reproduction and the development of neurodegenerative diseases.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD220 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to human CD220 - conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P06213
Entrez Gene
INSR
GO Terms
GO:0000187 activation of MAPK activity
GO:0001934 positive regulation of protein phosphorylation
GO:0003007 heart morphogenesis
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005009 insulin receptor activity
GO:0005159 insulin-like growth factor receptor binding
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0005792 microsome
GO:0005899 insulin receptor complex
GO:0005901 caveola
GO:0005975 carbohydrate metabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010008 endosome membrane
GO:0046326 positive regulation of glucose import
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0019087 transformation of host cell by virus
GO:0030335 positive regulation of cell migration
GO:0031994 insulin-like growth factor I binding
GO:0031995 insulin-like growth factor II binding
GO:0032148 activation of protein kinase B activity
GO:0032583 regulation of gene-specific transcription
GO:0042169 SH2 domain binding
GO:0042593 glucose homeostasis
GO:0043410 positive regulation of MAPKKK cascade
GO:0043548 phosphatidylinositol 3-kinase binding
GO:0043559 insulin binding
GO:0043560 insulin receptor substrate binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0045995 regulation of embryonic development
GO:0046777 protein autophosphorylation
GO:0048639 positive regulation of developmental growth
GO:0051290 protein heterotetramerization
GO:0051425 PTB domain binding
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0060267 positive regulation of respiratory burst
Regulatory
For research purposes only

Applications of CD220 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 15ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas, Islet of Langerhans
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for CD220 antibody

  1. Youngren, J.F. (2007) Regulation of insulin receptor function.
    Cell Mol Life Sci. 64 (7-8): 873-91.
  2. Wada, A. et al. (2005) New twist on neuronal insulin receptor signaling in health, disease, and therapeutics.
    J Pharmacol Sci. 99 (2): 128-43.
  3. Toth, B. et al. (2009) Insulin and leptin receptors as possible new candidates for endocrine control in normal and disturbed human pregnancy.
    Mol Hum Reprod. 15 (4): 231-9.

Further Reading

  1. Plum, L. et al. (2005) The role of insulin receptor signaling in the brain.
    Trends Endocrinol Metab. 16 (2): 59-65.

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