Rabbit anti Human CD220 antibody detects an epitope within in the internal domain of human CD220, also known as Insulin receptor (INRS). CD220 is a heterotetrameric membrane glycoprotein consisting of four disulfide linked subunits; two alpha subunits and two beta subunits. CD220 is a member of the tyrosine kinase family of receptors, and mediates the effects of insulin and IGF-I (insulin-like growth factor-I) through the activation of insulin receptor substrate (IRS) proteins and downstream effector cascades.
Activation of CD220 leads to a number of cellular effects, including glucose uptake, the regulation of cell proliferation and gene expression. These effects mostly occur in skeletal muscle, fat and the liver. Neuronal CD220 signalling has been shown to have an important role in energy homeostasis, reproduction and the development of neurodegenerative diseases.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CD220 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide corresponding to human CD220 - conjugated to KLH.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
positive regulation of protein phosphorylation
receptor signaling protein tyrosine kinase activity
insulin receptor activity
insulin-like growth factor receptor binding
metal ion binding
insulin receptor complex
carbohydrate metabolic process
G-protein coupled receptor protein signaling pathway
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
positive regulation of glucose import
transformation of host cell by virus
positive regulation of cell migration
insulin-like growth factor I binding
insulin-like growth factor II binding
activation of protein kinase B activity
regulation of gene-specific transcription
SH2 domain binding
positive regulation of MAPKKK cascade
phosphatidylinositol 3-kinase binding
insulin receptor substrate binding
positive regulation of nitric oxide biosynthetic process
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
positive regulation of glycolysis
positive regulation of mitosis
regulation of embryonic development
positive regulation of developmental growth
PTB domain binding
positive regulation of protein kinase B signaling cascade
positive regulation of respiratory burst
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD220 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Pancreas, Islet of Langerhans
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Secondary Antibodies Available
Useful Reagents Available