CD196 antibody

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Anti Human CD196 Antibody thumbnail image 2

Anti Human CD196 Antibody gallery image 1 — Paraffin embedded human spleen stained with Goat anti Human CD196 (**AHP1813**).

Anti Human CD196 Antibody gallery image 2 — Paraffin embedded mouse spleen stained with Goat anti Human CD196 (**AHP1813**).

Goat anti Human CD196

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1813	E P WB			0.1 mg

Goat anti Human CD196 antibody recognizes human CD196, otherwise known as CCR6 (C-C chemokine receptor type 6), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which acts as a specific receptor for the C-C chemokine LARC (Liver and activation-regulated chemokine)/MIP-3alpha, a selective chemoattractant which induces calcium flux in CD196-expressing lymphocytes.

CD196 is preferentially expressed by B cells, memory T cells and immature dendritic cells and plays an important role in B lineage maturation and antigen-driven B cell differentiation. CD196 acts as a co-receptor for HIV1 infection, and is selectively expressed by mucosa-associated non-Hodgkin's lymphoma, making the interaction between CD196 and LARC a potential therapeutic target in these malignancies.

Goat anti Human CD196 antibody cross-reacts with mouse CD196, where linear epitopes are accessible.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human CD196 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)
0.1% Bovine Serum Albumin

Immunogen

Synthetic peptide YFVSVNTSYYSVDSEMLLCSLQEVRQFSR corresponding to amino acids 18-46 of human CD196.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P51684 Related reagents; O54689 Related reagents
Entrez Gene: Ccr6 Related reagents; CCR6 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0016493 C-C chemokine receptor activity; GO:0005887 integral to plasma membrane; GO:0006928 cellular component movement; GO:0006935 chemotaxis; GO:0006959 humoral immune response; GO:0006968 cellular defense response; GO:0007204 elevation of cytosolic calcium ion concentration
Regulatory: For research purposes only

Applications of CD196 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/35000
Immunohistology - Paraffin			1/125
Western Blotting			1/250

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue: Spleen

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

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