CD196 antibody

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Goat anti Human CD196

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1813 E P WB 0.1 mg
Goat anti Human CD196 antibody recognizes human CD196, otherwise known as CCR6 (C-C chemokine receptor type 6), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which acts as a specific receptor for the C-C chemokine LARC (Liver and activation-regulated chemokine)/MIP-3alpha, a selective chemoattractant which induces calcium flux in CD196-expressing lymphocytes.

CD196 is preferentially expressed by B cells, memory T cells and immature dendritic cells and plays an important role in B lineage maturation and antigen-driven B cell differentiation. CD196 acts as a co-receptor for HIV1 infection, and is selectively expressed by mucosa-associated non-Hodgkin's lymphoma, making the interaction between CD196 and LARC a potential therapeutic target in these malignancies.

Goat anti Human CD196 antibody cross-reacts with mouse CD196, where linear epitopes are accessible.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CD196 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide YFVSVNTSYYSVDSEMLLCSLQEVRQFSR corresponding to amino acids 18-46 of human CD196.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P51684 Related reagents
O54689 Related reagents
Entrez Gene
Ccr6 Related reagents
CCR6 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0016493 C-C chemokine receptor activity
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0006935 chemotaxis
GO:0006959 humoral immune response
GO:0006968 cellular defense response
GO:0007204 elevation of cytosolic calcium ion concentration
Regulatory
For research purposes only

Applications of CD196 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/35000
Immunohistology - Paraffin 1/125
Western Blotting 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue
Spleen

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P