CD195 antibody

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Goat anti CD195

CD195 antibody recognizes C-C chemokine receptor type 5, also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, or HIV-1 fusion coreceptor. The antibody has been extensively validated for western blotting using whole cell lysates.

CD195 Antibody with Control Lysate

CD195 antibody recognizes C-C chemokine receptor type 5, also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, or HIV-1 fusion coreceptor. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00044 WB 100 µl
VPA00044KT WB 2 Westerns
With Lysate 400µg
Goat anti Human CD195 antibody recognizes human CD195, also known as C-C chemokine receptor type 5, CCR5 or C-C CKR-5. CD195 is a 353 amino acid ~37 kDa multiple pass transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by a subset of memory Th1 (type 1 helper T) cells.

CD195 acts as a high-affinity receptor for several chemotactic chemokines, including MIP-1 alpha (macrophage inflammatory protein 1 alpha), MIP-1 beta, and RANTES (regulated on activation normal T expressed and secreted protein). CD195 is also a major co-receptor for HIV-1 infection, with variations in the CD195 gene, influencing the rate of progression into AIDS (Choe et al. 1996).

Goat anti Human CD195 antibody detects CD195 as a 37 kDa band, in a number of human cell lysates, by western blotting under reducing conditions. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
100μl Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5 % Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5 % Bovine Serum Albumin
Immunogen
Synthetic peptide YQVSSPIYDINYYTSEPCQKINVKQIAA corresponding to amino acids 3-30 within the N-terminal region of human CD195.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P51681 Related reagents
Entrez Gene
CCR5 Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD195 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.
Western Blotting
Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB