CD192 antibody

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Anti Human CD192 (N-Terminal) Antibody thumbnail image 1

Anti Human CD192 (N-Terminal) Antibody thumbnail image 2

Anti Human CD192 (N-Terminal) Antibody gallery image 1 — Paraffin embedded human spleen stained with Goat anti Human CD192 (N-Terminal) (**AHP1810**).

Anti Human CD192 (N-Terminal) Antibody gallery image 2 — **Published customer image:**
Goat anti Human CD192 antibody (**AHP1810**) used to assess CCR2 expression in rat spinal cord tissue by western blotting.
**Image caption:**
Expression and localization of CCR2 after spinal cord injury. (A, B) Western blotting detection of CCR2 expression in spinal cord tissue.

From: Rong Y, Ji C, Wang Z, Ge X, Wang J, Ye W, Tang P, Jiang D, Fan J, Yin G, Liu W, Cai W.
Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 2021 Sep 12;18(1):196.
doi: 10.1186/s12974-021-02268-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Human CD192 (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1810 Datasheet	E P WB SDS	0.1 mg	Log in		Get Quote

Goat anti Human CD192 antibody recognizes an epitope within the N-terminal (NT) region of human CD192, otherwise known as CCR2 (C-C chemokine receptor type 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which acts as a receptor for the chemokines MCP-1 (monocyte chemoattractant protein 1), MCP-3 and MCP-4.

Interaction between CD192 and MCP-1 initiates chemotactic responses, through an increase in intracellular calcium levels. CD192 acts as a co-receptor with CD4 for HIV1 infection, and variations in CD192 are associated with relative resistance to HIV1.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human CD192 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
0.1% Bovine Serum Albumin
Immunogen: Synthetic peptide C-GEEVTTFFDYDYGAPCHKFDVKQ corresponding to amino acids 17-39 within the N-terminal region of human CD192.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P41597
Entrez Gene: CCR2
GO Terms: GO:0005887 integral to plasma membrane; GO:0005625 soluble fraction; GO:0005737 cytoplasm; GO:0006935 chemotaxis; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0006968 cellular defense response; GO:0007194 negative regulation of adenylate cyclase activity; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0007259 JAK-STAT cascade; GO:0016493 C-C chemokine receptor activity; GO:0019221 cytokine-mediated signaling pathway; GO:0031727 CCR2 chemokine receptor binding; GO:0044419 interspecies interaction between organisms
Regulatory: For research purposes only

Applications of CD192 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/30000
Immunohistology - Paraffin			1/100
Western Blotting			1/500

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue: Spleen.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Product Specific References

References for CD192 antibody

Rong, Y. et al. (2021) Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 18 (1): 196.

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