CD184 / CXCR4 antibody

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Goat anti Human CD184 / CXCR4

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1808	E P WB			0.1 mg

Goat anti Human CD184 / CXCR4 antibody recognises an epitope within the N-terminal (NT) region of human CD184, otherwise known as CXCR4 (C-X-C chemokine receptor type 4), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, ubiquitously expressed in blood and tissue cells, which acts as a specific receptor for the chemokine SDF-1 (Stromal cell-derived factor 1).

Interaction of CD184 with SDF-1 mediates the chemotaxis of progenitor and mature blood cells, and is critical for B- lymphopoiesis and myelopoiesis. CD184 is a major co-receptor for infection by T-cell tropic strains of HIV1, and also a primary receptor for some HIV2 isolates.

This Goat anti Human CD184 / CXCR4 antibody is reported as being suitable for use in immunofluorescence studies.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human CD184 / CXCR4 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)
0.1% Bovine Serum Albumin

Immunogen

Synthetic peptide TEEMGSGDYDSMKEPCFREENANFNKC corresponding to amino acids 17-43 within the N-terminal region of human CD184 / CXCR4.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P61073 Related reagents; O08565 Related reagents
Entrez Gene: Cxcr4 Related reagents; CXCR4 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0001764 neuron migration; GO:0002064 epithelial cell development; GO:0016021 integral to membrane; GO:0005768 endosome; GO:0008038 neuron recognition; GO:0016493 C-C chemokine receptor activity; GO:0016494 C-X-C chemokine receptor activity; GO:0022029 telencephalon cell migration; GO:0043067 regulation of programmed cell death; GO:0050920 regulation of chemotaxis; GO:0051924 regulation of calcium ion transport; GO:0060048 cardiac muscle contraction; GO:0000187 activation of MAPK activity; GO:0001666 response to hypoxia; GO:0003779 actin binding; GO:0009615 response to virus; GO:0006915 apoptosis; GO:0006954 inflammatory response; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0009986 cell surface; GO:0015026 coreceptor activity; GO:0016023 cytoplasmic membrane-bounded vesicle; GO:0019059 initiation of viral infection; GO:0030260 entry into host cell; GO:0031252 cell leading edge; GO:0031625 ubiquitin protein ligase binding; GO:0032027 myosin light chain binding; GO:0043130 ubiquitin binding
Regulatory: For research purposes only

Applications of CD184 / CXCR4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/25000
Immunohistology - Paraffin			1/150
Western Blotting			1/250

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Spleen

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

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