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CD156b antibody

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Anti Human CD156b (C-Terminal) Antibody thumbnail image 1 Anti Human CD156b (C-Terminal) Antibody thumbnail image 2
Anti Human CD156b (C-Terminal) Antibody gallery image 1
Western blot analysis of CD156b (TACE) in HeLa (1,3) and Jurkat (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide probed with Rabbit anti Human CD156b (AHP589).
Anti Human CD156b (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of HeLa cells with Rabbit anti Human CD156b (AHP589).

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WB IC

Rabbit anti Human CD156b (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD156b
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP589
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human CD156b antibody recognises the C-terminal region of the TNF alpha converting enzyme (TACE), designated CD156b at the 6th leukocyte typing workshop. TACE is a membrane-bound metalloprotease disintegrin belonging to the mammalian ADAM family.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD156b were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide corresponding to amino acids 807 to 823 of human CD156b
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Moss, M.L. et al. (1997) Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha.
    Nature. 385 (6618): 733-6.

Synonyms
ADAM-17
TACE
RRID
AB_323399
UniProt
P78536
Entrez Gene
ADAM17
GO Terms
GO:0001666 response to hypoxia
GO:0001934 positive regulation of protein phosphorylation
GO:0002446 neutrophil mediated immunity
GO:0002467 germinal center formation
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0005138 interleukin-6 receptor binding
GO:0005178 integrin binding
GO:0005737 cytoplasm
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007219 Notch signaling pathway
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0042493 response to drug
GO:0009986 cell surface
GO:0010820 positive regulation of T cell chemotaxis
GO:0015629 actin cytoskeleton
GO:0016324 apical plasma membrane
GO:0017124 SH3 domain binding
GO:0030165 PDZ domain binding
GO:0030183 B cell differentiation
GO:0030307 positive regulation of cell growth
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0032496 response to lipopolysaccharide
GO:0032717 negative regulation of interleukin-8 production
GO:0032722 positive regulation of chemokine production
GO:0033025 regulation of mast cell apoptosis
GO:0033077 T cell differentiation in thymus
GO:0033627 cell adhesion mediated by integrin
GO:0035313 wound healing, spreading of epidermal cells
GO:0035625 epidermal growth factor receptor transactivation by G-protein coupled receptor signaling pathway
GO:0045121 membrane raft
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048536 spleen development
GO:0051088 PMA-inducible membrane protein ectodomain proteolysis
GO:0055099 response to high density lipoprotein particle stimulus

AHP589

140414

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