CD156b antibody

100% Secure


Goat anti Human CD156b (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1071 E WB 0.1 mg
Goat anti Human CD156b antibody detects an epitope within the C-terminus of isoform 1 of CD156b, a ~125 kDa type I transmembrane glycoprotein which belongs to the ADAM (a disintegrin and metalloprotease domain) family. CD156b functions as a tumour necrosis factor-alpha converting enzyme (TACE). CD156b also causes a number of inflammatory modulators to undergo ectodomain shedding, including TNFR75, IL-1RII, TNFR55, L-selectin, and the amyloid precursor protein among others. CD156b plays a prominent role in the activation of the Notch signalling pathway.

ADAM17 plays a pivotal role in several acquired tumor cell capabilities by mediating the availability of soluble transforming growth factor-alpha, and establishing an autocrine signaling pathway through endogenous EGFR activation. CD156b is therefore a prospective therapeutic target in human cancer.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken Expected from Sequence
Hamster Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD156b were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence LQRQNRVDSKETEC from the C-terminus of CD156b.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P78536 Related reagents
Entrez Gene
ADAM17 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0001934 positive regulation of protein phosphorylation
GO:0002446 neutrophil mediated immunity
GO:0002467 germinal center formation
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0005138 interleukin-6 receptor binding
GO:0005178 integrin binding
GO:0005737 cytoplasm
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007219 Notch signaling pathway
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0042493 response to drug
GO:0009986 cell surface
GO:0010820 positive regulation of T cell chemotaxis
GO:0015629 actin cytoskeleton
GO:0016324 apical plasma membrane
GO:0017124 SH3 domain binding
GO:0030165 PDZ domain binding
GO:0030183 B cell differentiation
GO:0030307 positive regulation of cell growth
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0032496 response to lipopolysaccharide
GO:0032717 negative regulation of interleukin-8 production
GO:0032722 positive regulation of chemokine production
GO:0033025 regulation of mast cell apoptosis
GO:0033077 T cell differentiation in thymus
GO:0033627 cell adhesion mediated by integrin
GO:0035313 wound healing, spreading of epidermal cells
GO:0035625 epidermal growth factor receptor transactivation by G-protein coupled receptor signaling pathway
GO:0045121 membrane raft
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048536 spleen development
GO:0051088 PMA-inducible membrane protein ectodomain proteolysis
GO:0055099 response to high density lipoprotein particle stimulus
Regulatory
For research purposes only

Applications of CD156b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Western Blotting 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1071 detects a band of approximately 125-130kDa in HeLa, Jurkat and U937 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for CD156b antibody

  1. Walcheck, B. et al. (2006) ADAM17 activity during human neutrophil activation and apoptosis.
    Eur J Immunol. 36 (4): 968-76.
  2. Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses.
    Vet Immunol Immunopathol. 129: 66-75.