CD154 antibody
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P ResetGoat anti Human CD154
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD154
Antibody Quality and Performance Guarantee
| Goat anti Human CD154 antibody recognizes the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154. CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Yeast - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human CD154 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Immunogen
- Recombinant human CD154
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
2.5 | 5.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Purified goat anti human CD154 may be used in indirect ELISA with recombinant human CD154 (PHP126) as the standard.
- Histology Positive Control Tissue
- Human cervical squamous cell carcinoma
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Further Reading
-
Hollenbaugh, D. et al. (1992) The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.
EMBO J. 11 (12): 4313-21.
- Synonyms
- CD40 Ligand
- RRID
- AB_609580
- UniProt
- P29965
- Entrez Gene
- CD40LG
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005174 CD40 receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0030168 platelet activation
- GO:0032735 positive regulation of interleukin-12 production
- GO:0042100 B cell proliferation
- GO:0045190 isotype switching
View more products with CD154 specificity
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