Rabbit anti CD140a (pTyr849)
recognizes the CD140a also known as platelet-derived growth factor receptor alpha (PDGFRA), when phosphorylated at tyrosine 849. CD140a belongs to the tyrosine protein kinase family and plays an important role in embryonic development, the cell cycle and chemotaxis.PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hart et al. 1990
). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hart et al. 1988
Upon ligand binding, CD140a is autophosphorylated at tyrosine 849 leading to an increase in CD140a activity (Zemskov et al. 2009
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to CD140a (pTyr849) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 849 of human CD140a
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor receptor activity
integral to plasma membrane
platelet-derived growth factor receptor binding
positive regulation of cell migration
protein homodimerization activity
positive regulation of DNA replication
positive regulation of fibroblast proliferation
platelet-derived growth factor binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD140a (pTyr849) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Hart, C.E. et al. (1990) Purification of PDGF-AB and PDGF-BB from human platelet extracts and identification of all three PDGF dimers in human platelets.
Biochemistry 29 (1): 166-72.
Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
Science 240 (4858): 1529-31.
Zemskov, E.A. et al. (2009) Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
J Biol Chem. 284 (24): 16693-703.
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