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CD140a (pTyr849) antibody

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Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Rabbit anti CD140a (pTyr849) antibody (AHP2568). Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

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Rabbit anti CD140a (pTyr849)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD140a (pTyr849)

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Product Code Applications Pack Size List Price Your Price Qty
AHP2568
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
List Price Your Price
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Rabbit anti CD140a (pTyr849) recognizes the CD140a also known as platelet-derived growth factor receptor alpha (PDGFRA), when phosphorylated at tyrosine 849. CD140a belongs to the tyrosine protein kinase family and plays an important role in embryonic development, the cell cycle and chemotaxis.

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hart et al. 1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hart et al. 1988).

Upon ligand binding, CD140a is autophosphorylated at tyrosine 849 leading to an increase in CD140a activity (Zemskov et al. 2009).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CD140a (pTyr849) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 849 of human CD140a
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Hart, C.E. et al. (1990) Purification of PDGF-AB and PDGF-BB from human platelet extracts and identification of all three PDGF dimers in human platelets.
    Biochemistry 29 (1): 166-72.
  2. Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
    Science 240 (4858): 1529-31.
  3. Zemskov, E.A. et al. (2009) Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
    J Biol Chem. 284 (24): 16693-703.

UniProt
P16234
Entrez Gene
PDGFRA
GO Terms
GO:0001775 cell activation
GO:0005018 platelet-derived growth factor alpha-receptor activity
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005161 platelet-derived growth factor receptor binding
GO:0005524 ATP binding
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030335 positive regulation of cell migration
GO:0042803 protein homodimerization activity
GO:0045740 positive regulation of DNA replication
GO:0046777 protein autophosphorylation
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding

AHP2568

141530

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