CD140a (pTyr754) antibody
Rabbit anti CD140a (pTyr754) recognizes the CD140a antigen also known as platelet-derived growth factor receptor alpha (PDGFRA), when phosphorylated at tyrosine 754.
CD140a belongs to the tyrosine protein kinase family and plays an important role in embryonic development, the cell cycle and chemotaxis. Phosphorylation at tyrosine 754 mediates activation of specific signal-transducing molecules thus initiating signal-tranduction pathways from the alpha beta receptor heterodimer, which are distinct from those initiated via homodimeric receptor complexes (Rupp et al. 1994).
CD140a belongs to the tyrosine protein kinase family and plays an important role in embryonic development, the cell cycle and chemotaxis. Phosphorylation at tyrosine 754 mediates activation of specific signal-transducing molecules thus initiating signal-tranduction pathways from the alpha beta receptor heterodimer, which are distinct from those initiated via homodimeric receptor complexes (Rupp et al. 1994).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to CD140a (pTyr754) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 754 of human CD140a
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P16234 Related reagents
- Entrez Gene
- PDGFRA Related reagents
- GO Terms
- GO:0001775 cell activation
- GO:0005018 platelet-derived growth factor alpha-receptor activity
- GO:0005021 vascular endothelial growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005524 ATP binding
- GO:0018108 peptidyl-tyrosine phosphorylation
- GO:0030335 positive regulation of cell migration
- GO:0042803 protein homodimerization activity
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048407 platelet-derived growth factor binding
- Regulatory
- For research purposes only
Applications of CD140a (pTyr754) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Rupp, E. et al. (1994) A unique autophosphorylation site in the platelet-derived growth factor alpha receptor from a heterodimeric receptor complex.
Eur J Biochem. 225 (1): 29-41.
