CD137 antibody
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Goat anti Human CD137 antibody specifically recognizes human CD137, otherwise known as 4-1BB ligand receptor, a type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily, predominantly expressed by activated T cells, which acts as a receptor for 4-1BB ligand (4-1BBL).
Interaction between CD137 and 4-1BBL, acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to act as a signal transducer of NF-kappaB activation.
Interaction between CD137 and 4-1BBL, acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to act as a signal transducer of NF-kappaB activation.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human CD137 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant human CD137
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q07011 Related reagents
- Entrez Gene
- TNFRSF9 Related reagents
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006917 induction of apoptosis
- GO:0008285 negative regulation of cell proliferation
- Regulatory
- For research purposes only
Applications of CD137 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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