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CD136 antibody

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Anti Human CD136 Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human colon surface epithelium with Rabbut anti Human CD136 (AHP1321).

Rabbit anti Human CD136

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1321
Datasheet
P*
SDS
50 µg loader

Rabbit anti Human CD136 antibody detects CD136, a receptor tyrosine kinase also known as Macrophage stimulating 1 receptor (MST1R) and RON (Recepteur d’origine nantais). CD136 is a member of the MET proto-oncogene family, and is the receptor for macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival.

CD136 is a disulfide-linked ~180 kDa heterodimer composed of a 40 kDa alpha chain and a 150 kDa beta chain, and is mainly expressed in cells of epithelial origin. In immune cells CD136 expression is restricted to certain types of tissue macrophages, and its activation is critical in modulating macrophage activities and preventing an excessive inflammatory response. CD136 is also required for embryonic development.

CD136 expression is significantly altered in several primary human cancers including those of the breast and colon.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD136 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide: internal domain of human CD136, conjugated to keyhole limpet hemocyanin.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q04912
Entrez Gene
MST1R
GO Terms
GO:0005515 protein binding
GO:0006952 defense response
GO:0005011 macrophage colony-stimulating factor receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006928 cellular component movement
GO:0007275 multicellular organismal development
GO:0007338 single fertilization
GO:0008284 positive regulation of cell proliferation
Regulatory
For research purposes only

Applications of CD136 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Colon, surface epithelium.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

Further Reading

  1. Wang, M.H. et al. (2002) Macrophage-stimulating protein and RON receptor tyrosine kinase: potential regulators of macrophage inflammatory activities.
    Scand J Immunol. 56 (6): 545-53.

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Request a different product with the CD136 specificity