CD120b (soluble) antibody
Rabbit anti Human CD120b (soluble) antibody, recognizes soluble CD120b, also known as TNF receptor type II, TNFRSF1B and p80 TNF-alpha receptor.
CD120b is a member of the TNF receptor superfamily and is a single spanning type I transmembrane receptor. Upon binding of its ligand TNF, the adaptor proteins TRAF-2 and TRAF-2-associated proteins are recruited. This results in the activation of the NF-κB signaling pathway as well as the activation of other signaling kinases (Wajant & Siegmund 2019). TNF-CD120b signaling promotes cell proliferation and survival (Ahmad et al. 2018).
A soluble form of CD120b has been described and is produced as a result of alternative splicing or shedding. The soluble form competes with the membrane bound form for the ligand TNF. The binding of TNF to soluble CD120b effectively inhibits TNF signaling by competing with the membrane bound form (Wajant & Siegmund 2019).
CD120b is a member of the TNF receptor superfamily and is a single spanning type I transmembrane receptor. Upon binding of its ligand TNF, the adaptor proteins TRAF-2 and TRAF-2-associated proteins are recruited. This results in the activation of the NF-κB signaling pathway as well as the activation of other signaling kinases (Wajant & Siegmund 2019). TNF-CD120b signaling promotes cell proliferation and survival (Ahmad et al. 2018).
A soluble form of CD120b has been described and is produced as a result of alternative splicing or shedding. The soluble form competes with the membrane bound form for the ligand TNF. The binding of TNF to soluble CD120b effectively inhibits TNF signaling by competing with the membrane bound form (Wajant & Siegmund 2019).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
- Antiserum Preparation
- Produced from sera of rabbits immunized with recombinant human sTNF-receptor type II and purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human soluble CD120b comprising the cysteine-rich, ligand binding portion of the extracellular domain of the CD120b protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 0.1 ng/μg of protein (< 1 EU/μg)
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20333
- Entrez Gene
- TNFRSF1B
- GO Terms
- GO:0005886 plasma membrane
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005031 tumor necrosis factor receptor activity
- GO:0045121 membrane raft
- Regulatory
- For research purposes only
Applications of CD120b (soluble) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 ug/ml | 2.0 ug/ml | |
| Western Blotting | 0.1 ug/ml | 0.2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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