CD120b (soluble) antibody

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Rabbit anti Human CD120b (soluble):Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2799Z
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Human CD120b (soluble) antibody, recognizes soluble CD120b, also known as TNF receptor type II, TNFRSF1B and p80 TNF-alpha receptor.

CD120b is a member of the TNF receptor superfamily and is a single spanning type I transmembrane receptor. Upon binding of its ligand TNF, the adaptor proteins TRAF-2 and TRAF-2-associated proteins are recruited. This results in the activation of the NF-κB signaling pathway as well as the activation of other signaling kinases (Wajant & Siegmund 2019). TNF-CD120b signaling promotes cell proliferation and survival (Ahmad et al. 2018).

A soluble form of CD120b has been described and is produced as a result of alternative splicing or shedding. The soluble form competes with the membrane bound form for the ligand TNF. The binding of TNF to soluble CD120b effectively inhibits TNF signaling by competing with the membrane bound form (Wajant & Siegmund 2019).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant human sTNF-receptor type II and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant human soluble CD120b comprising the cysteine-rich, ligand binding portion of the extracellular domain of the CD120b protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P20333
Entrez Gene
TNFRSF1B
GO Terms
GO:0005886 plasma membrane
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005031 tumor necrosis factor receptor activity
GO:0045121 membrane raft
Regulatory
For research purposes only

Applications of CD120b (soluble) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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