CCT Alpha antibody

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Goat anti CCT Alpha

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00123
Datasheet
WB
MSDS
100 µl

Goat anti Human CCT alpha antibody recognizes the CCT alpha protein, also known as T-complex protein 1 subunit alpha (TCP-1-alpha).

CCT alpha, encoded by the TCP1 gene, is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of TCP1, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from TCP1 have been found (provided by RefSeq, Jun 2010).

Goat anti Human CCT alpha antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CCT alpha detects a band of approximately 60 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P17987
Entrez Gene
TCP1
GO Terms
GO:0051082 unfolded protein binding
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0007021 tubulin complex assembly
GO:0030054 cell junction
GO:0051084 'de novo' posttranslational protein folding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CCT Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for CCT Alpha antibody

  1. Yuen, G. et al. (2017) CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level.
    Nucleic Acids Res. 45 (20): 12039-53.