CBX5 antibody

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Rabbit anti CBX5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00452
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human CBX5 antibody recognizes CBX5, also known as heterochromatin protein 1 homolog alpha, HP1-alpha, chromobox protein homolog 5, antigen p25 or epididymis luminal protein 25.

The CBX5 gene encodes a highly conserved nonhistone protein, a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).

Rabbit anti Human CBX5 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CBX5 detects a band of approximately 26 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human CBX5

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P45973
Entrez Gene
CBX5
GO Terms
GO:0000118 histone deacetylase complex
GO:0006333 chromatin assembly or disassembly
GO:0030674 protein binding, bridging
GO:0007596 blood coagulation
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0016565 general transcriptional repressor activity
GO:0017053 transcriptional repressor complex
GO:0031618 nuclear centromeric heterochromatin
GO:0035064 methylated histone residue binding
GO:0035097 histone methyltransferase complex
GO:0044419 interspecies interaction between organisms
GO:0070491 repressing transcription factor binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of CBX5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml