CBX5 antibody
Rabbit anti CBX5
Rabbit anti-human CBX5 antibody recognizes chromobox protein homolog 5, also known as HP1-ALPHA, antigen p25, epididymis luminal protein 25 or heterochromatin protein 1-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.
CBX5 Antibody with Control Lysate
Rabbit anti-human CBX5 antibody recognizes chromobox protein homolog 5, also known as HP1-ALPHA, antigen p25, epididymis luminal protein 25 or heterochromatin protein 1-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00452 | WB | 100 µl | ||||
| VPA00452KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CBX5 antibody recognizes CBX5, also known as heterochromatin protein 1 homolog alpha, HP1-alpha, chromobox protein homolog 5, antigen p25 or epididymis luminal protein 25.
The CBX5 gene encodes a highly conserved nonhistone protein, a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).
Rabbit anti Human CBX5 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The CBX5 gene encodes a highly conserved nonhistone protein, a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).
Rabbit anti Human CBX5 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CBX5 detects a band of approximately 26 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
- Preparation
- 20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the middle region of human CBX5
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P45973 Related reagents
- Entrez Gene
- CBX5 Related reagents
- GO Terms
- GO:0000118 histone deacetylase complex
- GO:0006333 chromatin assembly or disassembly
- GO:0030674 protein binding, bridging
- GO:0007596 blood coagulation
- GO:0005635 nuclear envelope
- GO:0005737 cytoplasm
- GO:0005730 nucleolus
- GO:0016565 general transcriptional repressor activity
- GO:0017053 transcriptional repressor complex
- GO:0031618 nuclear centromeric heterochromatin
- GO:0035064 methylated histone residue binding
- GO:0035097 histone methyltransferase complex
- GO:0044419 interspecies interaction between organisms
- GO:0070491 repressing transcription factor binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CBX5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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