CBX5 antibody

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Rabbit anti CBX5

Rabbit anti-human CBX5 antibody recognizes chromobox protein homolog 5, also known as HP1-ALPHA, antigen p25, epididymis luminal protein 25 or heterochromatin protein 1-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.

CBX5 Antibody with Control Lysate

Rabbit anti-human CBX5 antibody recognizes chromobox protein homolog 5, also known as HP1-ALPHA, antigen p25, epididymis luminal protein 25 or heterochromatin protein 1-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00452 WB 100 µl
VPA00452KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CBX5 antibody recognizes CBX5, also known as heterochromatin protein 1 homolog alpha, HP1-alpha, chromobox protein homolog 5, antigen p25 or epididymis luminal protein 25.

The CBX5 gene encodes a highly conserved nonhistone protein, a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).

Rabbit anti Human CBX5 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CBX5 detects a band of approximately 26 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
Preparation
20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human CBX5
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P45973 Related reagents
Entrez Gene
CBX5 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0006333 chromatin assembly or disassembly
GO:0030674 protein binding, bridging
GO:0007596 blood coagulation
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0016565 general transcriptional repressor activity
GO:0017053 transcriptional repressor complex
GO:0031618 nuclear centromeric heterochromatin
GO:0035064 methylated histone residue binding
GO:0035097 histone methyltransferase complex
GO:0044419 interspecies interaction between organisms
GO:0070491 repressing transcription factor binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CBX5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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