CBX3 antibody
Goat anti CBX3
Goat anti-human CBX3 antibody recognizes chromobox protein homolog 3, also known as HP1 gamma homolog, heterochromatin protein HP1 gamma or heterochromatin protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
CBX3 Antibody with Control Lysate
Goat anti-human CBX3 antibody recognizes chromobox protein homolog 3, also known as HP1 gamma homolog, heterochromatin protein HP1 gamma or heterochromatin protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00435 | WB | 100 µl | ||||
| VPA00435KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human CBX3 antibody recognizes CBX3, also known as HP1 gamma homolog, heterochromatin protein 1 homolog gamma or heterochromatin protein HP1 gamma.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by CBX3 gene binds DNA and is a component of heterochromatin. This protein can also bind to the lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for CBX3 (provided by RefSeq, Mar 2011).
Goat anti Human CBX3 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by CBX3 gene binds DNA and is a component of heterochromatin. This protein can also bind to the lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for CBX3 (provided by RefSeq, Mar 2011).
Goat anti Human CBX3 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CBX3 detects a band of approximately 22 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-EAFLNSQKAGKEKD
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q13185 Related reagents
- Entrez Gene
- CBX3 Related reagents
- GO Terms
- GO:0000779 condensed chromosome, centromeric region
- GO:0003682 chromatin binding
- GO:0006333 chromatin assembly or disassembly
- GO:0005637 nuclear inner membrane
- GO:0005719 nuclear euchromatin
- GO:0005819 spindle
- GO:0006338 chromatin remodeling
- GO:0006350 transcription
- GO:0016565 general transcriptional repressor activity
- GO:0019899 enzyme binding
- GO:0019904 protein domain specific binding
- GO:0031618 nuclear centromeric heterochromatin
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CBX3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
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