CBX3 antibody

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Goat anti CBX3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1086 E WB 0.1 mg
Goat anti Human CBX3 antibody recognizes human chromobox homolog 3 (CBX3), a ~20 kDa protein. It binds histone H3 tails methylated at lysine 9, repressing transcription. CBX3 also interacts with lamin B receptor, forming a functional kinetochore through interaction with MIS12 complex proteins. This dual binding may contribute to the association of heterochromatin with the inner nuclear membrane.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Dog Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CBX3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence C-EAFLNSQKAGKEKD corresponding to the C-terminus of CBX (NP_009207.2; NP_057671.2).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13185 Related reagents
Entrez Gene
CBX3 Related reagents
GO Terms
GO:0000779 condensed chromosome, centromeric region
GO:0003682 chromatin binding
GO:0006333 chromatin assembly or disassembly
GO:0005637 nuclear inner membrane
GO:0005719 nuclear euchromatin
GO:0005819 spindle
GO:0006338 chromatin remodeling
GO:0006350 transcription
GO:0016565 general transcriptional repressor activity
GO:0019899 enzyme binding
GO:0019904 protein domain specific binding
GO:0031618 nuclear centromeric heterochromatin
Regulatory
For research purposes only

Applications of CBX3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Western Blotting 0.3 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1086 detects a band of approximately 20kDa in A431, Hela and HepG2 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Ye, Q. & Worman, H.J. (1996) Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.
    J Biol Chem. 271 (25): 14653-6.