Caveolin 1 (pTyr14) antibody

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Rabbit anti Caveolin 1 (pTyr14)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2565 WB 50 µl
Rabbit anti caveolin 1 (pTyr14) antibody recognizes caveolin 1 when phosphorylated at tyrosine 14. Caveolin 1 is a member of the caveolin family of proteins which are the main structural components of caveolae. Caveolin-1 has a number of functions, with important roles in cholesterol homeostasis, vesicle trafficking, signal transduction and tumorigenesis (Williams and Lisanti 2005).

In response to oxidative stress, caveolin-1 is phosphorylated by Src tyrosine kinase family members, Src, Abl or Fyn at tyrosine 14.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to caveolin 1 (pTyr14) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 14 of human caveolin 1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q03135 Related reagents
Entrez Gene
CAV1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0000188 inactivation of MAPK activity
GO:0005515 protein binding
GO:0001570 vasculogenesis
GO:0001666 response to hypoxia
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007596 blood coagulation
GO:0005102 receptor binding
GO:0005783 endoplasmic reticulum
GO:0005811 lipid particle
GO:0005901 caveola
GO:0006641 triglyceride metabolic process
GO:0006816 calcium ion transport
GO:0006940 regulation of smooth muscle contraction
GO:0007519 skeletal muscle tissue development
GO:0009267 cellular response to starvation
GO:0010524 positive regulation of calcium ion transport into cytosol
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015485 cholesterol binding
GO:0016050 vesicle organization
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016504 peptidase activator activity
GO:0019217 regulation of fatty acid metabolic process
GO:0019915 lipid storage
GO:0030193 regulation of blood coagulation
GO:0030514 negative regulation of BMP signaling pathway
GO:0030857 negative regulation of epithelial cell differentiation
GO:0031295 T cell costimulation
GO:0031623 receptor internalization
GO:0032091 negative regulation of protein binding
GO:0032507 maintenance of protein location in cell
GO:0032570 response to progesterone stimulus
GO:0032947 protein complex scaffold
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033484 nitric oxide homeostasis
GO:0042632 cholesterol homeostasis
GO:0051260 protein homooligomerization
GO:0043409 negative regulation of MAPKKK cascade
GO:0043627 response to estrogen stimulus
GO:0044419 interspecies interaction between organisms
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045907 positive regulation of vasoconstriction
GO:0046209 nitric oxide metabolic process
GO:0048471 perinuclear region of cytoplasm
GO:0048554 positive regulation of metalloenzyme activity
GO:0050900 leukocyte migration
GO:0050998 nitric-oxide synthase binding
GO:0050999 regulation of nitric-oxide synthase activity
GO:0051480 cytosolic calcium ion homeostasis
GO:0051592 response to calcium ion
GO:0051899 membrane depolarization
GO:0060056 mammary gland involution
GO:0070836 caveola assembly
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of Caveolin 1 (pTyr14) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Williams, T.M. & Lisanti, M.P. (2005) Caveolin-1 in oncogenic transformation, cancer, and metastasis
    Am J Physiol Cell Physiol. 288 (3): C494-506.