Caveolin 1 (pTyr14) antibody

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Rabbit anti Caveolin 1 (pTyr14)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2565	WB			50 µl

Rabbit anti caveolin 1 (pTyr14) antibody recognizes caveolin 1 when phosphorylated at tyrosine 14. Caveolin 1 is a member of the caveolin family of proteins which are the main structural components of caveolae. Caveolin-1 has a number of functions, with important roles in cholesterol homeostasis, vesicle trafficking, signal transduction and tumorigenesis (Williams and Lisanti 2005).

In response to oxidative stress, caveolin-1 is phosphorylated by Src tyrosine kinase family members, Src, Abl or Fyn at tyrosine 14.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to caveolin 1 (pTyr14) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding tyrosine 14 of human caveolin 1

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q03135 Related reagents
Entrez Gene: CAV1 Related reagents
GO Terms: GO:0000139 Golgi membrane; GO:0000188 inactivation of MAPK activity; GO:0005515 protein binding; GO:0001570 vasculogenesis; GO:0001666 response to hypoxia; GO:0001937 negative regulation of endothelial cell proliferation; GO:0007596 blood coagulation; GO:0005102 receptor binding; GO:0005783 endoplasmic reticulum; GO:0005811 lipid particle; GO:0005901 caveola; GO:0006641 triglyceride metabolic process; GO:0006816 calcium ion transport; GO:0006940 regulation of smooth muscle contraction; GO:0007519 skeletal muscle tissue development; GO:0009267 cellular response to starvation; GO:0010524 positive regulation of calcium ion transport into cytosol; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0015485 cholesterol binding; GO:0016050 vesicle organization; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0016504 peptidase activator activity; GO:0019217 regulation of fatty acid metabolic process; GO:0019915 lipid storage; GO:0030193 regulation of blood coagulation; GO:0030514 negative regulation of BMP signaling pathway; GO:0030857 negative regulation of epithelial cell differentiation; GO:0031295 T cell costimulation; GO:0031623 receptor internalization; GO:0032091 negative regulation of protein binding; GO:0032507 maintenance of protein location in cell; GO:0032570 response to progesterone stimulus; GO:0032947 protein complex scaffold; GO:0033137 negative regulation of peptidyl-serine phosphorylation; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0033484 nitric oxide homeostasis; GO:0042632 cholesterol homeostasis; GO:0051260 protein homooligomerization; GO:0043409 negative regulation of MAPKKK cascade; GO:0043627 response to estrogen stimulus; GO:0044419 interspecies interaction between organisms; GO:0045019 negative regulation of nitric oxide biosynthetic process; GO:0045907 positive regulation of vasoconstriction; GO:0046209 nitric oxide metabolic process; GO:0048471 perinuclear region of cytoplasm; GO:0048554 positive regulation of metalloenzyme activity; GO:0050900 leukocyte migration; GO:0050998 nitric-oxide synthase binding; GO:0050999 regulation of nitric-oxide synthase activity; GO:0051480 cytosolic calcium ion homeostasis; GO:0051592 response to calcium ion; GO:0051899 membrane depolarization; GO:0060056 mammary gland involution; GO:0070836 caveola assembly; GO:0090090 negative regulation of canonical Wnt receptor signaling pathway; GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory: For research purposes only

Applications of Caveolin 1 (pTyr14) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*