Cathepsin H antibody

Rabbit anti Cathepsin H

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Cathepsin H

Product Code Applications Pack Size List Price Your Price Qty
AHP2708
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
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Search for Batch Specific Datasheets

Rabbit anti Cathepsin H antibody recognizes pro-cathepsin H, also known as CTSH and CPSB. Cathepsin H is a lysosomal cysteine protease that is synthesized as an inactive proenzyme composed of prodomain and catalytic domains. It is currently unclear if the processing/cleavage of the proenzyme is performed by an autocatalytic method (Rojnik et al. 2012) or requires other enzymes for its activation (Hao et al. 2018). Cathepsin H has been reported to be overexpressed in human prostate tumor cells (Waghray et al. 2002).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to cathepsin H was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
50% Glycerol
Immunogen
Synthesized peptide derived from the internal region of human cathepsin H (amino acids 70-150)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti cathepsin H antibody detects a band of approximately 40 kDa in HEK 293 cell lysate

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UniProt
P09668
Entrez Gene
CTSH
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0005764 lysosome
GO:0006508 proteolysis
GO:0031648 protein destabilization
View more products with CATHEPSIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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