Rabbit anti Cathepsin H antibody
recognizes pro-cathepsin H, also known as CTSH and CPSB. Cathepsin H is a lysosomal cysteine protease that is synthesized as an inactive proenzyme composed of prodomain and catalytic domains. It is currently unclear if the processing/cleavage of the proenzyme is performed by an autocatalytic method (Rojnik et al. 2012
) or requires other enzymes for its activation (Hao et al. 2018
). Cathepsin H has been reported to be overexpressed in human prostate tumor cells (Waghray et al. 2002
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to cathepsin H was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Synthesized peptide derived from the internal region of human cathepsin H (amino acids 70-150)
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cysteine-type endopeptidase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cathepsin H antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti cathepsin H antibody detects a band of approximately 40 kDa in HEK 293 cell lysate
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Secondary Antibodies Available
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