Cathepsin B antibody

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Sheep anti Human Cathepsin B:FITC

Sheep anti Human Cathepsin B:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1910-8000F E 1 ml
1910-8000P E 1 ml
Sheep anti Human cathepsin B antibody detects cathepsin B, a lysosomal cysteine protease which functions as both a endopeptidase and a carboxypeptidase. Together with cathepsins D and L, cathepsin B is one of the most abundant proteases in the lysosome, and participates in intracellular protein degradation.

Cathepsin B is thought to play an increasingly important role in apoptosis and its release from the lysosome under cell stress leads to Bid cleavage, cytochrome C release and nuclear fragmentation.

Cathepsin B is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete processing of APP is thought to be a causative factor in Alzheimer disease.

Over-expression of cathepsin B has been associated with oesophageal adenocarcinoma and other tumours, and has in particular been implicated in tumour invasion and metastasis.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Immunogen
Highly pure cathepsin B from human placenta.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07858 Related reagents
Entrez Gene
CTSB Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0005764 lysosome
GO:0006508 proteolysis
GO:0042981 regulation of apoptosis
GO:0042470 melanosome
GO:0050790 regulation of catalytic activity
Regulatory
For research purposes only

Applications of Cathepsin B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
ELISA 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E