Catenin Gamma antibody

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Goat anti Catenin Gamma

Gamma-catenin antibody recognizes junction plakoglobin, also known as catenin gamma desmoplakin III or desmoplakin-3. The antibody has been extensively validated for western blotting using whole cell lysates.

Catenin Gamma Antibody with Control Lysate

Gamma-catenin antibody recognizes junction plakoglobin, also known as catenin gamma desmoplakin III or desmoplakin-3. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00115 WB 100 µl
VPA00115KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human catenin gamma antibody recognizes catenin gamma, also known as the junction plakoglobin, desmoplakin III or desmoplakin-3.

Catenin gamma, encoded by the JUP gene, is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in JUP has been associated with Naxos disease. Alternative splicing occurs in JUP; however, not all transcripts have been fully described (provided by RefSeq, Jul 2008).

Mouse anti Human catenin gamma antibody detects a band of 82 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti catenin gamma detects a band of approximately 82 kDa in A431 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A431 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P14923 Related reagents
Entrez Gene
JUP Related reagents
GO Terms
GO:0000902 cell morphogenesis
GO:0003136 negative regulation of heart induction by canonical Wnt receptor signaling pathway
GO:0003181 atrioventricular valve morphogenesis
GO:0003308 negative regulation of Wnt receptor signaling pathway involved in heart development
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005102 receptor binding
GO:0005199 structural constituent of cell wall
GO:0005634 nucleus
GO:0005915 zonula adherens
GO:0005916 fascia adherens
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007369 gastrulation
GO:0007398 ectoderm development
GO:0007399 nervous system development
GO:0009898 internal side of plasma membrane
GO:0015629 actin cytoskeleton
GO:0016323 basolateral plasma membrane
GO:0016331 morphogenesis of embryonic epithelium
GO:0016342 catenin complex
GO:0016477 cell migration
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0030018 Z disc
GO:0030057 desmosome
GO:0032993 protein-DNA complex
GO:0034332 adherens junction organization
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0042153 RPTP-like protein binding
GO:0042307 positive regulation of protein import into nucleus
GO:0042803 protein homodimerization activity
GO:0043588 skin development
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0048599 oocyte development
GO:0050982 detection of mechanical stimulus
GO:0051091 positive regulation of transcription factor activity
GO:0071603 endothelial cell-cell adhesion
GO:0071665 gamma-catenin-TCF7L2 complex
GO:0071681 cellular response to indole-3-methanol
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Catenin Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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