Catenin Beta (pSer33) antibody

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Rabbit anti Catenin Beta (pSer33)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2563 WB 50 µl
Rabbit anti catenin beta (pSer33) antibody recognizes catenin beta, when phosphorylated at serine 33. Catenin beta is a component of adherens junctions protein complexes that are involved in cell adhesion and actin cytoskeleton organization. Catenin beta also plays an important role in the canonical Wnt signaling pathway.

Catenin beta is phosphorylated by either GSK-3β or homeodomain-interacting protein kinase 2 (HIPK2) at serine 33, leading to the degradation of catenin beta (Sadot et al. 2002, Kim et al. 2010).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to catenin beta (pSer33) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 33 of human catenin beta

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P35222 Related reagents
Entrez Gene
CTNNB1 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0000578 embryonic axis specification
GO:0001569 patterning of blood vessels
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0001702 gastrulation with mouth forming second
GO:0001711 endodermal cell fate commitment
GO:0001837 epithelial to mesenchymal transition
GO:0001889 liver development
GO:0003136 negative regulation of heart induction by canonical Wnt receptor signaling pathway
GO:0003337 mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0005198 structural molecule activity
GO:0005667 transcription factor complex
GO:0005813 centrosome
GO:0005915 zonula adherens
GO:0005916 fascia adherens
GO:0005924 cell-substrate adherens junction
GO:0005938 cell cortex
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007160 cell-matrix adhesion
GO:0007268 synaptic transmission
GO:0007398 ectoderm development
GO:0007403 glial cell fate determination
GO:0008022 protein C-terminus binding
GO:0008285 negative regulation of cell proliferation
GO:0042493 response to drug
GO:0009898 internal side of plasma membrane
GO:0009950 dorsal/ventral axis specification
GO:0009954 proximal/distal pattern formation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0010909 positive regulation of heparan sulfate proteoglycan biosynthetic process
GO:0014010 Schwann cell proliferation
GO:0016328 lateral plasma membrane
GO:0016337 cell-cell adhesion
GO:0016342 catenin complex
GO:0016564 transcription repressor activity
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0022009 central nervous system vasculogenesis
GO:0030018 Z disc
GO:0030027 lamellipodium
GO:0030057 desmosome
GO:0030331 estrogen receptor binding
GO:0030521 androgen receptor signaling pathway
GO:0030539 male genitalia development
GO:0030902 hindbrain development
GO:0030997 regulation of centriole-centriole cohesion
GO:0031016 pancreas development
GO:0031069 hair follicle morphogenesis
GO:0031528 microvillus membrane
GO:0032331 negative regulation of chondrocyte differentiation
GO:0032355 response to estradiol stimulus
GO:0032993 protein-DNA complex
GO:0033077 T cell differentiation in thymus
GO:0034333 adherens junction assembly
GO:0034394 protein localization at cell surface
GO:0034742 APC-Axin-1-beta-catenin complex
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0042129 regulation of T cell proliferation
GO:0042153 RPTP-like protein binding
GO:0042475 odontogenesis of dentine-containing tooth
GO:0043065 positive regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043198 dendritic shaft
GO:0043410 positive regulation of MAPKKK cascade
GO:0043587 tongue morphogenesis
GO:0044325 ion channel binding
GO:0044334 canonical Wnt receptor signaling pathway involved in positive regulation of epithelial to mesenchymal transition
GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
GO:0045202 synapse
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0045669 positive regulation of osteoblast differentiation
GO:0045671 negative regulation of osteoclast differentiation
GO:0045743 positive regulation of fibroblast growth factor receptor signaling pathway
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0048145 regulation of fibroblast proliferation
GO:0048262 determination of dorsal/ventral asymmetry
GO:0048471 perinuclear region of cytoplasm
GO:0048489 synaptic vesicle transport
GO:0048538 thymus development
GO:0048599 oocyte development
GO:0048617 embryonic foregut morphogenesis
GO:0048660 regulation of smooth muscle cell proliferation
GO:0050681 androgen receptor binding
GO:0050808 synapse organization
GO:0051145 smooth muscle cell differentiation
GO:0051149 positive regulation of muscle cell differentiation
GO:0060440 trachea formation
GO:0060479 lung cell differentiation
GO:0060484 lung-associated mesenchyme development
GO:0060492 lung induction
GO:0060769 positive regulation of epithelial cell proliferation involved in prostate gland development
GO:0060789 hair follicle placode formation
GO:0060916 mesenchymal cell proliferation involved in lung development
GO:0061047 positive regulation of branching involved in lung morphogenesis
GO:0061154 endothelial tube morphogenesis
GO:0070369 beta-catenin-TCF7L2 complex
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
GO:0070602 regulation of centromeric sister chromatid cohesion
GO:0071363 cellular response to growth factor stimulus
GO:0071681 cellular response to indole-3-methanol
GO:0072033 renal vesicle formation
GO:0072053 renal inner medulla development
GO:0072054 renal outer medulla development
GO:0072079 nephron tubule formation
GO:0090279 regulation of calcium ion import
GO:2000008 regulation of protein localization at cell surface
Regulatory
For research purposes only

Applications of Catenin Beta (pSer33) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Sadot, E. et al. (2002) Regulation of S33/S37 phosphorylated beta-catenin in normal and transformed cells
    J Cell Sci. 115 (Pt 13): 2771-80.
  2. Kim, E.A. et al. (2010) Homeodomain-interacting protein kinase 2 (HIPK2) targets beta-catenin for phosphorylation and proteasomal degradation
    Biochem Biophys Res Commun. 394 (4): 966-71.