Catenin Beta antibody

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Anti Catenin Beta Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Catenin Beta Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Catenin Beta Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human catenin beta 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to catenin beta 1 (molecular weight 90 kDa)

Anti Catenin Beta Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of Catenin beta knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti Catenin Beta antibody (**VPA00164**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 29 second and (B) 30 second exposure.

Anti Catenin Beta Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of Catenin Beta from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti Catenin Beta

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00164 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human catenin beta antibody recognizes the catenin beta, also known as beta-catenin.

Catenin beta is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Catenin beta also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Catenin beta binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in catenin beta are a cause of colorectal cancer, pilomatrixoma (PTR), medulloblastoma (MDB) and ovarian cancer. Three transcript variants encoding the same protein have been found for catenin beta (provided by RefSeq, Oct 2009).

Rabbit anti Human catenin beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti catenin beta detects a band of approximately 90 kDa in HEK293 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding to the C terminus of catenin beta

Approx. Protein Concentrations

IgG concentration 1.15 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P35222
Entrez Gene: CTNNB1
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0005515 protein binding; GO:0000578 embryonic axis specification; GO:0001569 patterning of blood vessels; GO:0001658 branching involved in ureteric bud morphogenesis; GO:0001702 gastrulation with mouth forming second; GO:0001711 endodermal cell fate commitment; GO:0001837 epithelial to mesenchymal transition; GO:0001889 liver development; GO:0003136 negative regulation of heart induction by canonical Wnt receptor signaling pathway; GO:0003337 mesenchymal to epithelial transition involved in metanephros morphogenesis; GO:0003704 specific RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0004871 signal transducer activity; GO:0005198 structural molecule activity; GO:0005667 transcription factor complex; GO:0005813 centrosome; GO:0005915 zonula adherens; GO:0005916 fascia adherens; GO:0005924 cell-substrate adherens junction; GO:0005938 cell cortex; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007016 cytoskeletal anchoring at plasma membrane; GO:0007160 cell-matrix adhesion; GO:0007268 synaptic transmission; GO:0007398 ectoderm development; GO:0007403 glial cell fate determination; GO:0008022 protein C-terminus binding; GO:0008285 negative regulation of cell proliferation; GO:0042493 response to drug; GO:0009898 internal side of plasma membrane; GO:0009950 dorsal/ventral axis specification; GO:0009954 proximal/distal pattern formation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010843 promoter binding; GO:0010909 positive regulation of heparan sulfate proteoglycan biosynthetic process; GO:0014010 Schwann cell proliferation; GO:0016328 lateral plasma membrane; GO:0016337 cell-cell adhesion; GO:0016342 catenin complex; GO:0016564 transcription repressor activity; GO:0019901 protein kinase binding; GO:0019903 protein phosphatase binding; GO:0022009 central nervous system vasculogenesis; GO:0030018 Z disc; GO:0030027 lamellipodium; GO:0030057 desmosome; GO:0030331 estrogen receptor binding; GO:0030521 androgen receptor signaling pathway; GO:0030539 male genitalia development; GO:0030902 hindbrain development; GO:0030997 regulation of centriole-centriole cohesion; GO:0031016 pancreas development; GO:0031069 hair follicle morphogenesis; GO:0031528 microvillus membrane; GO:0032331 negative regulation of chondrocyte differentiation; GO:0032355 response to estradiol stimulus; GO:0032993 protein-DNA complex; GO:0033077 T cell differentiation in thymus; GO:0034333 adherens junction assembly; GO:0034394 protein localization at cell surface; GO:0034742 APC-Axin-1-beta-catenin complex; GO:0034747 Axin-APC-beta-catenin-GSK3B complex; GO:0042129 regulation of T cell proliferation; GO:0042153 RPTP-like protein binding; GO:0042475 odontogenesis of dentine-containing tooth; GO:0043065 positive regulation of apoptosis; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043198 dendritic shaft; GO:0043410 positive regulation of MAPKKK cascade; GO:0043587 tongue morphogenesis; GO:0044325 ion channel binding; GO:0044334 canonical Wnt receptor signaling pathway involved in positive regulation of epithelial to mesenchymal transition; GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis; GO:0045202 synapse; GO:0045294 alpha-catenin binding; GO:0045296 cadherin binding; GO:0045669 positive regulation of osteoblast differentiation; GO:0045671 negative regulation of osteoclast differentiation; GO:0045743 positive regulation of fibroblast growth factor receptor signaling pathway; GO:0045765 regulation of angiogenesis; GO:0045768 positive regulation of anti-apoptosis; GO:0048145 regulation of fibroblast proliferation; GO:0048262 determination of dorsal/ventral asymmetry; GO:0048471 perinuclear region of cytoplasm; GO:0048489 synaptic vesicle transport; GO:0048538 thymus development; GO:0048599 oocyte development; GO:0048617 embryonic foregut morphogenesis; GO:0048660 regulation of smooth muscle cell proliferation; GO:0050681 androgen receptor binding; GO:0050808 synapse organization; GO:0051145 smooth muscle cell differentiation; GO:0051149 positive regulation of muscle cell differentiation; GO:0060440 trachea formation; GO:0060479 lung cell differentiation; GO:0060484 lung-associated mesenchyme development; GO:0060492 lung induction; GO:0060769 positive regulation of epithelial cell proliferation involved in prostate gland development; GO:0060789 hair follicle placode formation; GO:0060916 mesenchymal cell proliferation involved in lung development; GO:0061047 positive regulation of branching involved in lung morphogenesis; GO:0061154 endothelial tube morphogenesis; GO:0070369 beta-catenin-TCF7L2 complex; GO:0070411 I-SMAD binding; GO:0070412 R-SMAD binding; GO:0070602 regulation of centromeric sister chromatid cohesion; GO:0071363 cellular response to growth factor stimulus; GO:0071681 cellular response to indole-3-methanol; GO:0072033 renal vesicle formation; GO:0072053 renal inner medulla development; GO:0072054 renal outer medulla development; GO:0072079 nephron tubule formation; GO:0090279 regulation of calcium ion import; GO:2000008 regulation of protein localization at cell surface
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Catenin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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