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Catenin Beta antibody

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Western Blot Validated
Anti Catenin Beta Antibody (PrecisionAb™ Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human catenin beta 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to catenin beta 1 (molecular weight 90 kDa)

Rabbit anti Catenin Beta

beta-Catenin antibody recognizes the catenin beta-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Catenin Beta Antibody with Control Lysate

beta-Catenin antibody recognizes the catenin beta-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00164 WB 100 µl
VPA00164KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human catenin beta antibody recognizes the catenin beta, also known as beta-catenin.

Catenin beta is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Catenin beta also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Catenin beta binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in catenin beta are a cause of colorectal cancer, pilomatrixoma (PTR), medulloblastoma (MDB) and ovarian cancer. Three transcript variants encoding the same protein have been found for catenin beta (provided by RefSeq, Oct 2009).

Rabbit anti Human catenin beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti catenin beta detects a band of approximately 90 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the C terminus of catenin beta
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35222 Related reagents
Entrez Gene
CTNNB1 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0000578 embryonic axis specification
GO:0001569 patterning of blood vessels
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0001702 gastrulation with mouth forming second
GO:0001711 endodermal cell fate commitment
GO:0001837 epithelial to mesenchymal transition
GO:0001889 liver development
GO:0003136 negative regulation of heart induction by canonical Wnt receptor signaling pathway
GO:0003337 mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0005198 structural molecule activity
GO:0005667 transcription factor complex
GO:0005813 centrosome
GO:0005915 zonula adherens
GO:0005916 fascia adherens
GO:0005924 cell-substrate adherens junction
GO:0005938 cell cortex
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007160 cell-matrix adhesion
GO:0007268 synaptic transmission
GO:0007398 ectoderm development
GO:0007403 glial cell fate determination
GO:0008022 protein C-terminus binding
GO:0008285 negative regulation of cell proliferation
GO:0042493 response to drug
GO:0009898 internal side of plasma membrane
GO:0009950 dorsal/ventral axis specification
GO:0009954 proximal/distal pattern formation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0010909 positive regulation of heparan sulfate proteoglycan biosynthetic process
GO:0014010 Schwann cell proliferation
GO:0016328 lateral plasma membrane
GO:0016337 cell-cell adhesion
GO:0016342 catenin complex
GO:0016564 transcription repressor activity
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0022009 central nervous system vasculogenesis
GO:0030018 Z disc
GO:0030027 lamellipodium
GO:0030057 desmosome
GO:0030331 estrogen receptor binding
GO:0030521 androgen receptor signaling pathway
GO:0030539 male genitalia development
GO:0030902 hindbrain development
GO:0030997 regulation of centriole-centriole cohesion
GO:0031016 pancreas development
GO:0031069 hair follicle morphogenesis
GO:0031528 microvillus membrane
GO:0032331 negative regulation of chondrocyte differentiation
GO:0032355 response to estradiol stimulus
GO:0032993 protein-DNA complex
GO:0033077 T cell differentiation in thymus
GO:0034333 adherens junction assembly
GO:0034394 protein localization at cell surface
GO:0034742 APC-Axin-1-beta-catenin complex
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0042129 regulation of T cell proliferation
GO:0042153 RPTP-like protein binding
GO:0042475 odontogenesis of dentine-containing tooth
GO:0043065 positive regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043198 dendritic shaft
GO:0043410 positive regulation of MAPKKK cascade
GO:0043587 tongue morphogenesis
GO:0044325 ion channel binding
GO:0044334 canonical Wnt receptor signaling pathway involved in positive regulation of epithelial to mesenchymal transition
GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
GO:0045202 synapse
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0045669 positive regulation of osteoblast differentiation
GO:0045671 negative regulation of osteoclast differentiation
GO:0045743 positive regulation of fibroblast growth factor receptor signaling pathway
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0048145 regulation of fibroblast proliferation
GO:0048262 determination of dorsal/ventral asymmetry
GO:0048471 perinuclear region of cytoplasm
GO:0048489 synaptic vesicle transport
GO:0048538 thymus development
GO:0048599 oocyte development
GO:0048617 embryonic foregut morphogenesis
GO:0048660 regulation of smooth muscle cell proliferation
GO:0050681 androgen receptor binding
GO:0050808 synapse organization
GO:0051145 smooth muscle cell differentiation
GO:0051149 positive regulation of muscle cell differentiation
GO:0060440 trachea formation
GO:0060479 lung cell differentiation
GO:0060484 lung-associated mesenchyme development
GO:0060492 lung induction
GO:0060769 positive regulation of epithelial cell proliferation involved in prostate gland development
GO:0060789 hair follicle placode formation
GO:0060916 mesenchymal cell proliferation involved in lung development
GO:0061047 positive regulation of branching involved in lung morphogenesis
GO:0061154 endothelial tube morphogenesis
GO:0070369 beta-catenin-TCF7L2 complex
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
GO:0070602 regulation of centromeric sister chromatid cohesion
GO:0071363 cellular response to growth factor stimulus
GO:0071681 cellular response to indole-3-methanol
GO:0072033 renal vesicle formation
GO:0072053 renal inner medulla development
GO:0072054 renal outer medulla development
GO:0072079 nephron tubule formation
GO:0090279 regulation of calcium ion import
GO:2000008 regulation of protein localization at cell surface
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Catenin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
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