Caspase-7 antibody

Rabbit anti Caspase-7 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-7
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1788
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P * WB 0.1 mg loader
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Rabbit anti Caspase-7 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of Caspase-7, a ~35 kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3).

Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and granzyme-B mediated apoptosis pathways.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Caspase-7 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3).
Immunogen
A 16 amino acid peptide from human Caspase-7 amino-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Skeletal muscle.
Western Blotting
Rabbit anti human caspase-7 antibody detects a band of approximately 30kDa in human skeletal muscle cell lysate.

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Further Reading

  1. Duan, H. et al. (1996) ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.
    J Biol Chem. 271 (3): 1621-5.
  2. Juan, T.S. et al. (1997) Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3.
    Genomics. 40 (1): 86-93.
  3. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
    Biochem J. 326 ( Pt 1): 1-16.

Synonyms
ICE-LAP3
RRID
AB_2068005
UniProt
P55210
P97864
Entrez Gene
CASP7
Casp7
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0005789 endoplasmic reticulum membrane
GO:0006508 proteolysis
GO:0006921 cellular component disassembly involved in apoptosis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0031966 mitochondrial membrane
GO:0001836 release of cytochrome c from mitochondria
GO:0006917 induction of apoptosis
GO:0007507 heart development
GO:0009411 response to UV
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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