Caspase-7 antibody

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Rabbit anti Caspase-7 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1788 P* WB 0.1 mg
Rabbit anti Caspase-7 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of Caspase-7, a ~35 kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3).

Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and granzyme-B mediated apoptosis pathways.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Caspase-7 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3).
Immunogen
A 16 amino acid peptide from human Caspase-7 amino-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P55210 Related reagents
P97864 Related reagents
Entrez Gene
CASP7 Related reagents
Casp7 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0005789 endoplasmic reticulum membrane
GO:0006508 proteolysis
GO:0006921 cellular component disassembly involved in apoptosis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0031966 mitochondrial membrane
GO:0001836 release of cytochrome c from mitochondria
GO:0006917 induction of apoptosis
GO:0007507 heart development
GO:0009411 response to UV
Regulatory
For research purposes only

Applications of Caspase-7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Skeletal muscle.
Western Blotting
Rabbit anti human caspase-7 antibody detects a band of approximately 30kDa in human skeletal muscle cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Duan, H. et al. (1996) ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.
    J Biol Chem. 271 (3): 1621-5.
  2. Juan, T.S. et al. (1997) Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3.
    Genomics. 40 (1): 86-93.
  3. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
    Biochem J. 326 ( Pt 1): 1-16.