Caspase-5 antibody
Rabbit anti Human Caspase-5 antibody recognizes an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.
Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.
The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis (Wang et al. 1996).
Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.
The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis (Wang et al. 1996).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- A 14 amino acid peptide from the centre of human Caspase-5.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
Applications of Caspase-5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1.0ug/ml | 2.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP965 detects a band of approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8kDa)
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Wang, S. et al. (1996) Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.
J Biol Chem. 271 (34): 20580-7. -
Lin, X.Y. et al. (2000) Expression analysis of the human caspase-1 subfamily reveals specific regulation of the CASP5 gene by lipopolysaccharide and interferon-gamma.
J Biol Chem. 275 (51): 39920-6. -
Munday, N.A. et al. (1995) Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
J Biol Chem. 270 (26): 15870-6. -
Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
Biochem J. 326 ( Pt 1): 1-16.
