Caspase-5 antibody

Rabbit anti Human Caspase-5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-5

Product Code Applications Pack Size List Price Your Price Qty
AHP965
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Rabbit anti Human Caspase-5 antibody recognizes an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.

Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.

The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro cleavage of caspase-5 by the serine protease granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis (Wang et al. 1996).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 14 amino acid peptide from the centre of human Caspase-5.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1.0ug/ml 2.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP965 detects a band of approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8kDa)

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Further Reading

  1. Wang, S. et al. (1996) Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.
    J Biol Chem. 271 (34): 20580-7.
  2. Lin, X.Y. et al. (2000) Expression analysis of the human caspase-1 subfamily reveals specific regulation of the CASP5 gene by lipopolysaccharide and interferon-gamma.
    J Biol Chem. 275 (51): 39920-6.
  3. Munday, N.A. et al. (1995) Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
    J Biol Chem. 270 (26): 15870-6.
  4. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
    Biochem J. 326 ( Pt 1): 1-16.

Synonyms
ICH-3
RRID
AB_2244017
UniProt
P51878
Entrez Gene
CASP5
View more products with CASPASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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