Caspase-3 (Active) antibody
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WB ResetRabbit anti Caspase-3 (Active)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Caspase-3 (Active)
Antibody Quality and Performance Guarantee
| Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3 as the name implies is a member of the cysteine-aspartic acid protease family. Caspase-3 gets cleaved from an inactive 32 kDa zymogen into p17 and p12 chains. These two subunits dimerize to form the active caspase 3 protein, which acts as an effector caspase that cleaves and activates caspase-6, 7 and 9. Via this process caspase-3 plays an important role in mediating apoptosis. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
5mM EDTA
0.03% Proclin 300 - Immunogen
- Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
Store at -20°C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
20 | 40 ug/ml |
| Western Blotting | |
0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti caspase -3 recognizes the p17 fragment of the active caspase-3 (approximately 17 kDa) in lysate from Jurkat cells treated with 2 μM camptothecin.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- Synonyms
- Apopain
- CPP32
- UniProt
- P42574
- Entrez Gene
- CASP3
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0004197 cysteine-type endopeptidase activity
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0006508 proteolysis
- GO:0008624 induction of apoptosis by extracellular signals
- View More GO Terms
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008635 activation of caspase activity by cytochrome c
- GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
- GO:0034612 response to tumor necrosis factor
- GO:0043066 negative regulation of apoptosis
- GO:0048011 nerve growth factor receptor signaling pathway
AHP2286
154461
154840
157786
171218
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CASPASE specificity
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