Caspase-3 (Active) antibody

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Rabbit anti Caspase-3 (Active)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2286 P WB 50 µg
Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3 as the name implies is a member of the cysteine-aspartic acid protease family. Caspase-3 gets cleaved from an inactive 32 kDa zymogen into p17 and p12 chains. These two subunits dimerize to form the active caspase 3 protein, which acts as an effector caspase that cleaves and activates caspase-6, 7 and 9. Via this process caspase-3 plays an important role in mediating apoptosis.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P42574 Related reagents
Entrez Gene
CASP3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006508 proteolysis
GO:0008624 induction of apoptosis by extracellular signals
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
GO:0034612 response to tumor necrosis factor
GO:0043066 negative regulation of apoptosis
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of Caspase-3 (Active) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 20 40 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti caspase -3 recognizes the p17 fragment of the active caspase-3 (approximately 17 kDa) in lysate from Jurkat cells treated with 2 µM camptothecin

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB