Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3 as the name implies is a member of the cysteine-aspartic acid protease family. Caspase-3 gets cleaved from an inactive 32 kDa zymogen into p17 and p12 chains. These two subunits dimerize to form the active caspase 3 protein, which acts as an effector caspase that cleaves and activates caspase-6, 7 and 9. Via this process caspase-3 plays an important role in mediating apoptosis.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cysteine-type endopeptidase activity
DNA fragmentation involved in apoptotic nuclear change
induction of apoptosis by extracellular signals
induction of apoptosis by intracellular signals
activation of caspase activity by cytochrome c
nuclear fragmentation involved in apoptotic nuclear change
response to tumor necrosis factor
negative regulation of apoptosis
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Caspase-3 (Active) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti caspase -3 recognizes the p17 fragment of the active caspase-3 (approximately 17 kDa) in lysate from Jurkat cells treated with 2 µM camptothecin
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Secondary Antibodies Available