Caspase-14 antibody

Rabbit anti Caspase-14 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-14
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1175
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SDS Safety Datasheet SDS
WB 0.1 mg loader
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Rabbit anti Human Cspase-14 antibody recognizes the C-terminal (CT) region of human caspase-14; a ~27 kDa cysteine protease which is activated by zymogen processing during cellular differentiation. It is highly expressed by keratinocytes and trophoblast cells of the placenta, with lower expression in the choroid plexus, the pigmented layer of the retina, and hair follicles.

Expression of the caspase-14 gene has recently been identified in epithelial tumours, including cervical and bladder carcinoma. The biological function of caspase-14 in tumour and normal tissue remains to be classified.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Caspase-14 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide from near the carboxy-terminus of human Caspase-14.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1175 detects a band of approximately 27kDa in Jurkat cell lysates.

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Further Reading

  1. Park, K. et al. (2006) Expression and characterization of constitutively active human caspase-14.
    Biochem Biophys Res Commun. 347 (4): 941-8.
  2. Koenig, U. et al. (2005) Aberrant expression of caspase-14 in epithelial tumors.
    Biochem Biophys Res Commun. 335 (2): 309-13.
  3. White, L. et al. (2007) Caspase-14: a new player in cytotrophoblast differentiation.
    Reprod Biomed Online. 14 (3): 300-7.

RRID
AB_844584
UniProt
P31944
Entrez Gene
CASP14
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006508 proteolysis
GO:0008544 epidermis development
GO:0030154 cell differentiation
View more products with CASPASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products