Caspase-14 antibody

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Rabbit anti Caspase-14 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1175
Datasheet
WB
MSDS
0.1 mg loader

Rabbit anti Human Cspase-14 antibody recognizes the C-terminal (CT) region of human caspase-14; a ~27 kDa cysteine protease which is activated by zymogen processing during cellular differentiation. It is highly expressed by keratinocytes and trophoblast cells of the placenta, with lower expression in the choroid plexus, the pigmented layer of the retina, and hair follicles.

Expression of the caspase-14 gene has recently been identified in epithelial tumours, including cervical and bladder carcinoma. The biological function of caspase-14 in tumour and normal tissue remains to be classified.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Caspase-14 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide from near the carboxy-terminus of human Caspase-14.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P31944 Related reagents
Entrez Gene
CASP14 Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006508 proteolysis
GO:0008544 epidermis development
GO:0030154 cell differentiation
Regulatory
For research purposes only

Applications of Caspase-14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1175 detects a band of approximately 27kDa in Jurkat cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Park, K. et al. (2006) Expression and characterization of constitutively active human caspase-14.
    Biochem Biophys Res Commun. 347 (4): 941-8.
  2. Koenig, U. et al. (2005) Aberrant expression of caspase-14 in epithelial tumors.
    Biochem Biophys Res Commun. 335 (2): 309-13.
  3. White, L. et al. (2007) Caspase-14: a new player in cytotrophoblast differentiation.
    Reprod Biomed Online. 14 (3): 300-7.