CARD11 antibody

Rabbit anti CARD11

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CARD11

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AHP2445
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SDS Safety Datasheet SDS
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Rabbit anti CARD11 antibody recognizes caspase recruitment domain-containing protein 11 (CARD11), also known as CARMA1. CARD11 is part of both the membrane associated guanylate kinase (MAGUK) family and the caspase recruitment domain (CARD) family. CARD11 is involved in NF-KappaB activation through interaction with signaling protein BCL10.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CARD11 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human CARD11
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti CARD11 antibody detects a band of approximately 133 kDa in Raji cell lysate and mouse tissue lysate under reducing conditions

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UniProt
Q9BXL7
Entrez Gene
CARD11
GO Terms
GO:0005886 plasma membrane
GO:0001819 positive regulation of cytokine production
GO:0004385 guanylate kinase activity
GO:0005829 cytosol
GO:0042981 regulation of apoptosis
GO:0031295 T cell costimulation
GO:0045121 membrane raft
GO:0050700 CARD domain binding
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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