Carbonic Anhydrase Ix antibody

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Rabbit anti Carbonic Anhydrase Ix

Carbonic anhydrase IX antibody recognizes carbonic anhydrase IX, also know as CA9 or carbonate dehydratase IX.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00813 WB 100 µl
Rabbit anti Human carbonic anhydrase IX antibody recognizes carbonic anhydrase IX, also know as carbonic anhydrase 9 (CA9) or carbonate dehydratase IX.

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12 (provided by RefSeq, Jun 2014).

Rabbit anti Human carbonic anhydrase IX antibody detects bands of 35-58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti carbonic anhydrase IX antibody recognizes bands of approximately 35-58 kDa in HT-29 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human carbonic anhydrase IX
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q16790 Related reagents
Entrez Gene
CA9 Related reagents
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0016021 integral to membrane
GO:0005730 nucleolus
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
GO:0031528 microvillus membrane
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Carbonic Anhydrase Ix antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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