Carbonic Anhydrase III antibody
Rabbit anti Carbonic Anhydrase III
Rabbit anti-human carbonic anhydrase III antibody recognizes carbonic anhydrase 3, also known as CA-III, HEL-S-167mP, carbonate dehydratase III or epididymis secretory sperm binding protein Li 167mP.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00602 | WB | 100 µl |
Rabbit anti Human carbonic Anhydrase III antibody recognizes carbonic anhydrase III, also known as CA3, CA-III, HEL-S-167mP, carbonate dehydratase III or epididymis secretory sperm binding protein Li 167mP.
Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns (provided by RefSeq, Oct 2008).
Rabbit anti Human carbonic anhydrase III antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.
Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns (provided by RefSeq, Oct 2008).
Rabbit anti Human carbonic anhydrase III antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti carbonic anhydrase III detects a band of approximately 29 kDa in human skeletal muscle tissue
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human carbonic anhydrase III
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P07451 Related reagents
- Entrez Gene
- CA3 Related reagents
- GO Terms
- GO:0004089 carbonate dehydratase activity
- GO:0005737 cytoplasm
- GO:0006730 one-carbon metabolic process
- GO:0008270 zinc ion binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Carbonic Anhydrase III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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