Carbonic Anhydrase III antibody

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Rabbit anti Carbonic Anhydrase III

Rabbit anti-human carbonic anhydrase III antibody recognizes carbonic anhydrase 3, also known as CA-III, HEL-S-167mP, carbonate dehydratase III or epididymis secretory sperm binding protein Li 167mP.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00602 WB 100 µl
Rabbit anti Human carbonic Anhydrase III antibody recognizes carbonic anhydrase III, also known as CA3, CA-III, HEL-S-167mP, carbonate dehydratase III or epididymis secretory sperm binding protein Li 167mP.

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns (provided by RefSeq, Oct 2008).

Rabbit anti Human carbonic anhydrase III antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti carbonic anhydrase III detects a band of approximately 29 kDa in human skeletal muscle tissue
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human carbonic anhydrase III
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P07451 Related reagents
Entrez Gene
CA3 Related reagents
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0005737 cytoplasm
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Carbonic Anhydrase III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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