CAPZB antibody

100% Secure


Goat anti Human CAPZB

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP707 WB 0.1 mg
Goat anti Human CAPZB antibody recognizes human F-actin capping protein muscle Z-line beta also known as CapZ beta or CAPZB. CAPZB is a member of the F-actin capping protein family involved in the growth regulation of actin filaments by binding to the barbed ends of fast growing actin filaments (Bai et al. 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Bovine Expected from Sequence
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CAPZB were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Synthetic peptide NDLVEALKRKQQC corresponding to the C-terminus of CAPZB (NP_004921).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P47756 Related reagents
Entrez Gene
CAPZB Related reagents
GO Terms
GO:0003779 actin binding
GO:0030036 actin cytoskeleton organization
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0006928 cellular component movement
GO:0008290 F-actin capping protein complex
GO:0051693 actin filament capping
GO:0071203 WASH complex
Regulatory
For research purposes only

Applications of CAPZB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5ug/ml 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP707 detects a band of approximately 28-30kDa in human liver extracts. A minimum incubation time of 1 hour is recommended for this antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for CAPZB antibody

  1. Masszi, A. et al. (2010) Fate-determining mechanisms in epithelial-myofibroblast transition: major inhibitory role for Smad3.
    J Cell Biol. 188 (3): 383-99.

Further Reading

  1. Barron-casella, E.A. et al. (1995) Sequence analysis and chromosomal localization of human Cap Z. Conserved residues within the actin-binding domain may link Cap Z to gelsolin/severin and profilin protein families.
    J Biol Chem. 270 (37): 21472-9.