Loading

CaMK II Alpha (pThr286) antibody

100% Secure
Anti CaMK II Alpha (pThr286) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with PMA using Rabbit anti CaMK II alpha (pThr286) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with PMA (200 nM) for 30 minutes

Rabbit anti CaMK II Alpha (pThr286)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2562 WB 50 µl
Rabbit anti CaMK II alpha (pThr286) antibody recognizes calcium/calmodulin-dependent protein kinase type II subunit alpha (CaMK II alpha) also known as CaM kinase II subunit alpha, when phosphorylated at threonine 286. CaMK II alpha is a member of the CAMK serine/threonine protein kinase family involved in the central nervous system, neurotransmitter release and synaptic plasticity.

CaMK II alpha is activated upon binding of calcium/calmodulin, resulting in autophosphorylation at threonine 286.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CaMK II alpha (pThr286) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 286 of human CaMK II alpha

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q9UQM7 Related reagents
Entrez Gene
CAMK2A Related reagents
GO Terms
GO:0005829 cytosol
GO:0004683 calmodulin-dependent protein kinase activity
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007268 synaptic transmission
GO:0030054 cell junction
GO:0030666 endocytic vesicle membrane
GO:0042734 presynaptic membrane
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060333 interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of CaMK II Alpha (pThr286) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Sign-up to receive our eNewsletters
View more products with CaMK II Alpha (pThr286) specificity