CaMK II Alpha (pThr286) antibody
Rabbit anti CaMK II alpha (pThr286) antibody recognizes calcium/calmodulin-dependent protein kinase type II subunit alpha (CaMK II alpha) also known as CaM kinase II subunit alpha, when phosphorylated at threonine 286. CaMK II alpha is a member of the CAMK serine/threonine protein kinase family involved in the central nervous system, neurotransmitter release and synaptic plasticity.
CaMK II alpha is activated upon binding of calcium/calmodulin, resulting in autophosphorylation at threonine 286.
CaMK II alpha is activated upon binding of calcium/calmodulin, resulting in autophosphorylation at threonine 286.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to CaMK II alpha (pThr286) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 286 of human CaMK II alpha
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9UQM7
- Entrez Gene
- CAMK2A
- GO Terms
- GO:0005829 cytosol
- GO:0004683 calmodulin-dependent protein kinase activity
- GO:0005516 calmodulin binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006468 protein phosphorylation
- GO:0007268 synaptic transmission
- GO:0030054 cell junction
- GO:0030666 endocytic vesicle membrane
- GO:0042734 presynaptic membrane
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0060333 interferon-gamma-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of CaMK II Alpha (pThr286) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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