Calreticulin Precursor antibody

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Rabbit anti Calreticulin Precursor

Calreticulin precursor antibody recognizes calreticulin, also known as CRP55, ERp60, HACBP, sicca syndrome antigen A (autoantigen Ro; calreticulin), calregulin, epididymis secretory sperm binding protein Li 99n, or grp60.

Calreticulin Precursor Antibody with Control Lysate

Calreticulin precursor antibody recognizes calreticulin, also known as CRP55, ERp60, HACBP, sicca syndrome antigen A (autoantigen Ro; calreticulin), calregulin, epididymis secretory sperm binding protein Li 99n, or grp60.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00165 WB 100 µl
VPA00165KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human calreticulin precursor antibody recognizes calreticulin precursor also known as CRP55, calregulin, endoplasmic reticulum resident protein 90 (ERp60) and grp60.

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors.

Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes (provided by RefSeq, Jul 2008).

Rabbit anti Human calreticulin precursor antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
<30% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
<30% Glycerol
Immunogen
Synthetic peptide corresponding to the C terminus of human calreticulin precursor protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P27797 Related reagents
Entrez Gene
CALR Related reagents
GO Terms
GO:0044183 protein binding involved in protein folding
GO:0051082 unfolded protein binding
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001849 complement component C1q binding
GO:0002502 peptide antigen assembly with MHC class I protein complex
GO:0003677 DNA binding
GO:0003729 mRNA binding
GO:0051087 chaperone binding
GO:0005829 cytosol
GO:0005178 integrin binding
GO:0051208 sequestering of calcium ion
GO:0005529 sugar binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005844 polysome
GO:0006611 protein export from nucleus
GO:0007050 cell cycle arrest
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0042981 regulation of apoptosis
GO:0016564 transcription repressor activity
GO:0050821 protein stabilization
GO:0017148 negative regulation of translation
GO:0018279 protein N-linked glycosylation via asparagine
GO:0022417 protein maturation by protein folding
GO:0031625 ubiquitin protein ligase binding
GO:0033144 negative regulation of steroid hormone receptor signaling pathway
GO:0042824 MHC class I peptide loading complex
GO:0042921 glucocorticoid receptor signaling pathway
GO:0043687 post-translational protein modification
GO:0045665 negative regulation of neuron differentiation
GO:0045740 positive regulation of DNA replication
GO:0045787 positive regulation of cell cycle
GO:0048387 negative regulation of retinoic acid receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0050681 androgen receptor binding
GO:0050766 positive regulation of phagocytosis
GO:0090398 cellular senescence
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Calreticulin Precursor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti calreticulin precursor detects a band of approximately 55 kDa in Jurkat cell lysates.
Western Blotting
Anti calreticulin precursor detects a band of approximately 55 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB