CAD antibody

Rabbit anti CAD

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
CAD

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VPA00639
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Rabbit anti Human CAD antibody recognizes the DNA fragmentation factor subunit beta, also known as DNA fragmentation factor 40 kDa subunit, caspase-activated DNase, DFF40, CPAN or caspase-activated nuclease.

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for DFFB but the biological validity of some of these variants has not been determined (provided by RefSeq, Sep 2013).

Rabbit anti Human CAD antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti CAD detects a band of approximately 46 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human CAD
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O76075
Entrez Gene
DFFB
GO Terms
GO:0004536 deoxyribonuclease activity
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0019899 enzyme binding
GO:0030263 apoptotic chromosome condensation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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