Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for DFFB but the biological validity of some of these variants has not been determined (provided by RefSeq, Sep 2013).
Rabbit anti Human CAD antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Rabbit anti CAD detects a band of approximately 46 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Synthetic peptide encompassing part of the middle region of human CAD
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- O76075 Related reagents
- Entrez Gene
- DFFB Related reagents
- GO Terms
- GO:0004536 deoxyribonuclease activity
- GO:0005829 cytosol
- GO:0035556 intracellular signal transduction
- GO:0005654 nucleoplasm
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0019899 enzyme binding
- GO:0030263 apoptotic chromosome condensation
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of CAD antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|
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