C5 antibody
Sheep anti human C5 polyclonal antibody recognizes complement component 5 (C5), a member of the complement system. The complement system consists of a tightly regulated network of proteins. Its activation leads to a sequential cascade of enzymatic reactions, resulting in opsonisation of the pathogens and their removal by phagocytes, as well as cell lysis. Three pathways have been described, the antibody-dependent pathway (classical pathway), the alternative pathway and the lectin activation pathway (Ehrnthaller 2011, Nesargikar 2012). The complement system plays a critical role in both innate and adaptive immune responses. It is also involved in tissue regeneration, tumor growth and some human pathologies (Sarma 2011).
Complement component 5 is the initiator of the effector terminal phase of the complement system. C5 convertase cleaves C5 into C5a, a potent anaphylatoxin, and C5b, ultimately resulting in the formation of the multimeric membrane attack complex (MAC) together with the other components of the terminal pathway (C5b-C9) (Noris 2013).
Sheep anti human polyclonal antibody gives a single arc when tested by immunoelectrophoresis (IEP) against human plasma. The identity has been confirmed by double diffusion against fresh human plasma and a known anti human C5.
Complement component 5 is the initiator of the effector terminal phase of the complement system. C5 convertase cleaves C5 into C5a, a potent anaphylatoxin, and C5b, ultimately resulting in the formation of the multimeric membrane attack complex (MAC) together with the other components of the terminal pathway (C5b-C9) (Noris 2013).
Sheep anti human polyclonal antibody gives a single arc when tested by immunoelectrophoresis (IEP) against human plasma. The identity has been confirmed by double diffusion against fresh human plasma and a known anti human C5.
Product Details
- Target Species
- Human
- Product Form
- Ig fraction - liquid
- Preparation
- Ig fraction prepared by ion exchange chromatography
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.1% E-amino-n-caproic acid (EACA) 0.01% Benzamidine 1 mM ethylenediaminetetraacetic acid (EDTA) - Immunogen
- Human C5, purified from plasma.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01031
- Entrez Gene
- C5
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004866 endopeptidase inhibitor activity
- GO:0005579 membrane attack complex
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008009 chemokine activity
- GO:0019835 cytolysis
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010760 negative regulation of macrophage chemotaxis
- GO:0090197 positive regulation of chemokine secretion
- Regulatory
- For research purposes only
Applications of C5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunodiffusion | |||
| Immunohistology - Paraffin | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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