C5 antibody

100% Secure


Sheep anti Human C5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2222-8354 ID P 1 ml
Sheep anti human C5 polyclonal antibody recognizes complement component 5 (C5), a member of the complement system. The complement system consists of a tightly regulated network of proteins. Its activation leads to a sequential cascade of enzymatic reactions, resulting in opsonisation of the pathogens and their removal by phagocytes, as well as cell lysis. Three pathways have been described, the antibody-dependent pathway (classical pathway), the alternative pathway and the lectin activation pathway (Ehrnthaller et al. 2011, Nesargikar et al. 2012). The complement system plays a critical role in both innate and adaptive immune responses. It is also involved in tissue regeneration, tumor growth and some human pathologies (Sarma et al. 2011).

Complement component 5 is the initiator of the effector terminal phase of the complement system. C5 convertase cleaves C5 into C5a, a potent anaphylatoxin, and C5b, ultimately resulting in the formation of the multimeric membrane attack complex (MAC) together with the other components of the terminal pathway (C5b-C9) (Noris et al. 2013).

Sheep anti human polyclonal antibody gives a single arc when tested by immunoelectrophoresis (IEP) against human plasma. The identity has been confirmed by double diffusion against fresh human plasma and a known anti human C5.

Product Details

Target Species
Human
Product Form
Ig fraction - liquid
Preparation
Ig fraction prepared by ion exchange chromatography
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.1%E-amino-n-caproic acid (EACA)
0.01%Benzamidine
1 mMethylenediaminetetraacetic acid (EDTA)
Immunogen
Human C5, purified from plasma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01031 Related reagents
Entrez Gene
C5 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0004866 endopeptidase inhibitor activity
GO:0005579 membrane attack complex
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008009 chemokine activity
GO:0019835 cytolysis
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010760 negative regulation of macrophage chemotaxis
GO:0090197 positive regulation of chemokine secretion
Regulatory
For research purposes only

Applications of C5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion
Immunohistology - Paraffin 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB