Find out more about A.I.den - our SearchBot

C1q antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human C1q Antibody thumbnail image 1 Anti Human C1q Antibody thumbnail image 2
Anti Human C1q Antibody gallery image 1
Published customer image:
Sheep anti Human C1q antibody (2221-5004P) used to evaluate C1q levels in plasma samples by ELISA.
Image caption:
Levels of factors of the CP and LP; plotted values are nonlog for ease of comprehension.
(A) Plasma levels of C1q, (B) MBL oligomer, (C) C-reactive protein, and (D) immunocomplex-CIC-C1q in dengue-infected individuals at different stages of infection (acute and convalescent) and in healthy subjects. All complement proteins were measured by ELISA. Values shown are means and standard deviations. The p-values of two-tailed t-tests on the log-transformed data are indicated above each analysis.

From: Nascimento EJM, Silva AM, Cordeiro MT, Brito CA, Gil LHVG, Braga-Neto U, et al. (2009)
Alternative Complement Pathway Deregulation Is Correlated with Dengue Severity.
PLoS ONE 4(8): e6782.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human C1q Antibody gallery image 2
Published customer image:
Sheep anti Human C1q antibody (2221-5004P) used as a detection reagent in an ELISA for complement binding and activation.
Image caption:
Complement binding by Fc mutants. Complement binding and activation was assessed by ELISA on Fc coated wells.
Serial dilutions of Fc from 10 μg/ml were coated onto maxi-sorb 96 ELISA plates at 100 μl/well in 0.05M carbonate buffer overnight at 4oC. Following washing with PBST (PBS-Tween-20 0.05%), plates were incubated with human serum diluted 1/100 in gelatin veronal buffer for 1h at room temperature. After washing in PBST, plates were incubated with a 1/250 dilution of sheep anti-human C1q-HRP (Serotec: 2221-5004P) in PBST supplemented with 0.5% w/v BSA for 2h. For detection of the C5b-9 binding mAb, plates were washed as before prior to incubation for 2h with a 1:500 dilution of anti-mouse IgG2b-HRP. Plates were then washed ten times in PBST before developing for HRP substrate 3,3’,5,5’-tetramethyl-benzidine dihydrochloride in phosphate-citrate buffer. Reactions were stopped with 25μl 2M H2SO4 and plates read on a plate reader at OD450nm. Duplicate experiments are shown.

From: Baksmeier C, Blundell P, Steckel J, Schultz V, Gu Q, Da Silva Filipe A, Kohl A, Linnington C, Lu D, Dell A, Haslam S, Wang J, Czajkowsky D, Goebels N, Pleass RJ.
Modified recombinant human IgG1-Fc is superior to natural intravenous immunoglobulin at inhibiting immune-mediated demyelination.
Immunology. 2021 Sep;164(1):90-105.
doi: 10.1111/imm.13341.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Filter by Application:

E

Sheep anti Human C1q:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
C1q

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
2221-5004P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 ml
List Price Your Price

Sheep anti Human C1q antibody recognizes human C1q, a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis (Troedson et al. 2013).

Sheep anti human C1q antibody may cross react with C1q in other species.

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to human C1q were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by protein G affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human C1q.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/400
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus

References for C1q antibody

  1. Nascimento, E.J. et al. (2009) Alternative complement pathway deregulation is correlated with dengue severity.
    PLoS One. 4:e6782
  2. Lewis, M.J. et al. (2007) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
    Mol Immunol. 45: 818-27.
  3. Jacobs, B.C. et al. (2003) Immunoglobulins inhibit pathophysiological effects of anti-GQ1b-positive sera at motor nerve terminals through inhibition of antibody binding.
    Brain. 126: 2220-34.
  4. Arduin, E. et al. (2015) Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a.
    Mol Immunol. 63 (2): 456-63.
  5. Kao, D. et al. (2015) A Monosaccharide Residue Is Sufficient to Maintain Mouse and Human IgG Subclass Activity and Directs IgG Effector Functions to Cellular Fc Receptors.
    Cell Rep. 13 (11): 2376-85.
  6. Gearing, D.P. et al. (2016) In Vitro and In Vivo Characterization of a Fully Felinized Therapeutic Anti-Nerve Growth Factor Monoclonal Antibody for the Treatment of Pain in Cats.
    J Vet Intern Med. 30 (4): 1129-37.
  7. Baksmeier, C. et al. (2021) Modified recombinant human IgG1-Fc is superior to natural intravenous immunoglobulin at inhibiting immune-mediated demyelination.
    Immunology. 164 (1): 90-105.

ELISA

Synonyms
Complement Component 1q
RRID
AB_2067259
UniProt
P02747
P02745
P02746
Entrez Gene
C1QA
C1QB
C1QC
GO Terms
GO:0005602 complement component C1 complex
GO:0006958 complement activation, classical pathway
GO:0007267 cell-cell signaling
GO:0045087 innate immune response
GO:0005576 extracellular region
GO:0030853 negative regulation of granulocyte differentiation
GO:0045650 negative regulation of macrophage differentiation

View more products with C1Q specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products