c-Myc (pThr358) antibody

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Rabbit anti c-Myc (pThr358)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
50 µl loader

Rabbit anti c-Myc (pThr358) antibody recognizes proto-oncogene c-Myc, also known as Class E basic helix-loop-helix protein 39, transcription factor p64 and avian myelocytomatosis viral oncogene homolog, when phosphorylated at threonine 358. c-Myc is a transcription factor and a member of the Myc family of basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors. c-Myc is involved in regulation of the cell cycle, cell differentiation and apoptosis.

c-Myc is phosphorylated at threonine 358 by PAK2, this negatively regulates c-Myc activity by inhibiting DNA binding (Huang et al. 2004).

Product Details

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to c-Myc (pThr358) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Phospho specific-peptide corresponding to residues surrounding threonine 358 of human c-Myc

Storage Information

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006879 cellular iron ion homeostasis
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0032204 regulation of telomere maintenance
GO:0070888 E-box binding
GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
For research purposes only

Applications of c-Myc (pThr358) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Huang, Z. et al. (2004) Negative control of the Myc protein by the stress-responsive kinase Pak2.
    Mol Cell Biol. 24 (4): 1582-94.

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