c-Myc (pSer373) antibody

100% Secure


Rabbit anti c-Myc (pSer373)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2582 WB 50 µl
Rabbit anti c-Myc (pSer373) antibody recognizes proto-oncogene c-Myc, also known as Class E basic helix-loop-helix protein 39, transcription factor p64 and avian myelocytomatosis viral oncogene homolog, when phosphorylated at serine 373. c-Myc is a transcription factor and a member of the Myc family of basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors. c-Myc is involved in regulation of the cell cycle, cell differentiation and apoptosis.

Dimerization of c-Myc with bHLH-Zip protein Max is necessary for transcriptional activity (Nilsson and Cleveland 2003). c-Myc is phosphorylated at serine 373 by PAK2, this inhibits the ability of c-Myc to dimerize with Max, negatively regulating the activity of c-Myc (Huang et al. 2004). PKC-zeta is also reported to phosphorylate c-Myc at serine 373 (Kim et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to c-Myc (pSer373) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 373 of human c-Myc

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P01106 Related reagents
Entrez Gene
MYC Related reagents
GO Terms
GO:0005515 protein binding
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006879 cellular iron ion homeostasis
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0032204 regulation of telomere maintenance
GO:0070888 E-box binding
GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
Regulatory
For research purposes only

Applications of c-Myc (pSer373) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Nilsson, J.A. & Cleveland, J.L. (2003) Myc pathways provoking cell suicide and cancer.
    Oncogene 22 (56): 9007-21.
  2. Huang, Z. et al. (2004) Negative control of the Myc protein by the stress-responsive kinase Pak2.
    Mol Cell Biol. 24 (4): 1582-94.
  3. Kim, J.Y. et al. (2013) c-Myc phosphorylation by PKCĪ¶ represses prostate tumorigenesis.
    Proc Natl Acad Sci U S A. 110 (16): 6418-23.