Rabbit anti c-Myc (pSer373) antibody
recognizes proto-oncogene c-Myc, also known as Class E basic helix-loop-helix protein 39, transcription factor p64 and avian myelocytomatosis viral oncogene homolog, when phosphorylated at serine 373. c-Myc is a transcription factor and a member of the Myc family of basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors. c-Myc is involved in regulation of the cell cycle, cell differentiation and apoptosis.
Dimerization of c-Myc with bHLH-Zip protein Max is necessary for transcriptional activity (Nilsson and Cleveland 2003
). c-Myc is phosphorylated at serine 373 by PAK2, this inhibits the ability of c-Myc to dimerize with Max, negatively regulating the activity of c-Myc (Huang et al. 2004
). PKC-zeta is also reported to phosphorylate c-Myc at serine 373 (Kim et al. 2013
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to c-Myc (pSer373) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 373 of human c-Myc
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
branching involved in ureteric bud morphogenesis
sequence-specific DNA binding transcription factor activity
regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
cell cycle arrest
response to drug
transcription activator activity
regulation of telomere maintenance
positive regulation of metanephric cap mesenchymal cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of c-Myc (pSer373) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available