Rabbit anti c-Met (pTyr1234/pTyr1235) antibody
recognizes c-Met, also known as, hepatocyte growth factor (HGF) receptor or scatter factor (SF) receptor, when phosphorylated at tyrosine 1234/tyrosine 1235. c-Met is the receptor for hepatocyte growth factor (HGF). Upon ligand binding, c-Met homodimerizes and is phosphorylated at tyrosine 1234 and tyrosine 1235. c-Met activation leads to signal transduction via downstream signaling pathways such as MAPK, JNK and PI3K/Akt (Trusolino et al. 2010
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to c-Met (pTyr1234/pTyr1235) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 1234/tyrosine 1235 of human c-Met
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
hepatocyte growth factor receptor activity
integral to plasma membrane
basal plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of c-Met (pTyr1234/pTyr1235) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Trusolino, L. et al. (2010) MET signalling: principles and functions in development, organ regeneration and cancer.
Nat Rev Mol Cell Biol. 11 (12): 834-48.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra