Rabbit anti c-Met (pTyr1234/pTyr1235) antibody
recognizes c-Met, also known as, hepatocyte growth factor (HGF) receptor or scatter factor (SF) receptor, when phosphorylated at tyrosine 1234/tyrosine 1235. c-Met is the receptor for hepatocyte growth factor (HGF). Upon ligand binding, c-Met homodimerizes and is phosphorylated at tyrosine 1234 and tyrosine 1235. c-Met activation leads to signal transduction via downstream signaling pathways such as MAPK, JNK and PI3K/Akt (Trusolino et al. 2010
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to c-Met (pTyr1234/pTyr1235) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 1234/tyrosine 1235 of human c-Met
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
hepatocyte growth factor receptor activity
integral to plasma membrane
basal plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of c-Met (pTyr1234/pTyr1235) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Trusolino, L. et al. (2010) MET signalling: principles and functions in development, organ regeneration and cancer.
Nat Rev Mol Cell Biol. 11 (12): 834-48.